Abstract 2879: Proteome changes in mouse prostate induced by oral administration of penta-O-galloyl-beta-D-glucose (PGG) suggest targets for cancer chemoprevention
| العنوان: | Abstract 2879: Proteome changes in mouse prostate induced by oral administration of penta-O-galloyl-beta-D-glucose (PGG) suggest targets for cancer chemoprevention |
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| المؤلفون: | Katai Nhkata, Ahamd Shaik, Junxuan Lu, Kwan Hyun Kim, Sung-Hoon Kim, Jinhui Zhang, Chengguo Xing, Joshua D. Liao, Lei Wang |
| المصدر: | Cancer Research. 70:2879-2879 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Cancer Research, medicine.medical_specialty, biology, business.industry, Cancer, Glutathione, medicine.disease_cause, medicine.disease, chemistry.chemical_compound, Fatty acid synthase, medicine.anatomical_structure, Endocrinology, Oncology, chemistry, Oral administration, Prostate, In vivo, Internal medicine, Proteome, medicine, biology.protein, Carcinogenesis, business |
| الوصف: | 1, 2, 3, 4, 6-penta-O-galloyl-β-D-glucose (PGG), a naturally occurring hydrolyzable gallotannin, has been shown by us and others to exhibit in vivo anti-cancer effects against prostate and breast cancer xenograft or lung cancer allograft when administrated by i.p injection or oral gavage. However, little is known of its safety for chronic chemoprevention use and virtually nothing is known of its in vivo target proteins. In the present study, we treated C57B6 mice with daily gavage of PGG from 7 weeks of age to 14 weeks of age with dosages of 1 and 2 mg per mouse (40-50 and 80-100mg/kg body weight). The dosage choices were made in reference to the efficacious anti-cancer dose of 20 mg/kg body weight. General health, body weights and food intake of mice were monitored. At necropsy, the organ weights were measured. Proteomic patterns in the prostate were profiled by 2D LC-MALDI-TOF/TOF analysis with iTRAQ labeling. Our results show that neither dose affected feed intake and body weight gain, but the 2 mg dose led to a statistically significant but very minor decrease of the weight of prostate and thymus. Proteome profiling by LC-MALDI-TOF/TOF identified 810 proteins from mouse prostates and 623 of them were identified with >95% confidence. Expression of 56 proteins was significantly modulated by either 1mg or 2mg PGG daily treatment, four different kinds of dose responses of proteins modulated by PGG were observed. Protein quantization results were validated by another mass spectrometry, ESI-Q-TOF. Selected proteins were further validated by Western blots and mRNA level by real-time PCR. The proteome profiles modulated by PGG include suppression of oncoproteins such as fatty acid synthase and induction of tumor suppressor proteins such as glutathione S-transferase M. Overall, the data show protein changes induced by tolerated dosages of PGG in the normal prostate are conducive for cancer chemoprevention. The chemopreventive efficacy of PGG is being evaluated in a primary carcinogenesis model. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2879. |
| تدمد: | 1538-7445 0008-5472 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::c2ef32186534548039a2d8af0c95958fTest https://doi.org/10.1158/1538-7445.am10-2879Test |
| رقم الانضمام: | edsair.doi...........c2ef32186534548039a2d8af0c95958f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15387445 00085472 |
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