The use of oxidative stress biomarkers in live animals (in vivo) to predict meat quality deterioration postmortem (in vitro) caused by changes in muscle biochemical components1
| العنوان: | The use of oxidative stress biomarkers in live animals (in vivo) to predict meat quality deterioration postmortem (in vitro) caused by changes in muscle biochemical components1 |
|---|---|
| المؤلفون: | David L. Hopkins, Paul Lewandowski, Alaa El-Din A. Bekhit, Khageswor Giri, Eric N. Ponnampalam, Tim Plozza, Joe L. Jacobs |
| المصدر: | Journal of Animal Science. 95:3012-3024 |
| بيانات النشر: | Oxford University Press (OUP), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Pathology, medicine.medical_specialty, Isoprostane, Thiobarbituric acid, medicine.medical_treatment, Biology, medicine.disease_cause, 03 medical and health sciences, chemistry.chemical_compound, Animal science, Lipid oxidation, Genetics, medicine, Aroma, chemistry.chemical_classification, Vitamin E, 0402 animal and dairy science, food and beverages, 04 agricultural and veterinary sciences, General Medicine, biology.organism_classification, 040201 dairy & animal science, 030104 developmental biology, chemistry, Feedlot, Animal Science and Zoology, Oxidative stress, Food Science, Polyunsaturated fatty acid |
| الوصف: | This study was conducted to determine whether circulating concentrations of blood isoprostanes can be used as an effective biomarker in lambs to predict degradation of color and/or lipid stability in meat. Lambs ( = 84) were fed diets of either lucerne pasture, annual ryegrass pasture, a commercial feedlot pellet, or a combination of annual ryegrass and feedlot pellet for 8 wk, including a 2-wk adaptation period. Blood isoprostane concentration at wk 0, 4, 6 or 8 of feeding was determined. Blood isoprostane concentration for each animal was then correlated with muscle biochemical components that impact color and/or lipid oxidative status during retail display. This included lipid oxidation levels in muscle assessed by thiobarbituric acid reactive substances and meat redness determined by a HunterLab colorimetric spectrometer. Lambs that consumed the commercial feedlot pellet had a lower muscle vitamin E level (< 0.01) and a greater level of -6 PUFA ( < 0.001) compared with lambs finished on annual ryegrass or lucerne. Lipid oxidation levels were greatest for lambs finished on the feedlot ration, lowest in lambs finished on the ryegrass diet, and intermediate for lambs finished on lucerne and ryegrass-feedlot combination ( < 0.01). After 8 wk of feeding, blood isoprostane concentration was positively correlated with lipid oxidation of meat displayed for 72 h in simulated retail conditions ( < 0.01). There was a negative linear relationship between isoprostane concentration and muscle vitamin E concentration ( = 0.07), lipid oxidation and muscle vitamin E concentration ( < 0.01) but a positive linear relationship between isoprostane concentration and muscle -6 PUFA ( < 0.001) or lipid oxidation and muscle -6 PUFA concentration ( < 0.001). Blood isoprostane concentration and lipid oxidation in meat were influenced by muscle vitamin E and -6 PUFA but not by -3 PUFA. There was no significant relationship observed between blood isoprostane concentration at 0, 4, 6 or 8 wk feeding vs. overall meat color (redness of meat) at 0 and 72 h of display, stored under simulated retail conditions. The results indicate that circulating blood isoprostane concentration can be a useful tool to predict the oxidative status of postmortem meat. Future work will examine the impact of this relationship on meat flavor/aroma deterioration post farm. |
| تدمد: | 1525-3163 0021-8812 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::be499ac6e6ffd5eb30cd80eb8883a370Test https://doi.org/10.2527/jas.2016.0887Test |
| رقم الانضمام: | edsair.doi...........be499ac6e6ffd5eb30cd80eb8883a370 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15253163 00218812 |
|---|