Endocrine, Stemness, Proliferative, and Proteolytic Properties of Alarm Cells in Ruby-Red-Fin Shark (Rainbow Shark), Epalzeorhynchos frenatum (Teleostei: Cyprinidae)
| العنوان: | Endocrine, Stemness, Proliferative, and Proteolytic Properties of Alarm Cells in Ruby-Red-Fin Shark (Rainbow Shark), Epalzeorhynchos frenatum (Teleostei: Cyprinidae) |
|---|---|
| المؤلفون: | Sara S. Abdel-Hakeem, Soha A. Soliman, Hanan H. Abd-Elhafeez, Abdelraheim Attaai, Abeera M. El-Sayed, Alaa Sayed Abou-Elhamd |
| المصدر: | Microscopy and Microanalysis. 27:1251-1264 |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | biology, Epidermis (botany), Chemistry, Acridine orange, biology.organism_classification, Molecular biology, Staining, Silver stain, chemistry.chemical_compound, Trichrome, Alkaline phosphatase, Instrumentation, Immunostaining, Rainbow shark |
| الوصف: | The current study investigated the morphological, histochemical, and immunohistochemical characteristics of alarm cells and their precursors in ruby-red-fin shark (rainbow shark), Epalzeorhynchos frenatum (Teleostei: Cyprinidae). Precursor alarm cells were shown to be small, cuboidal, pyramidal, or round in shape, with eosinophilic cytoplasm, resting on the basement membrane of the epidermis. The cells later elongated to become columnar in shape. Subsequently, they enlarged and became large oval-shaped cells. They then underwent shrinkage and vacuolation. The superficial alarm cells were collapsed. Alarm cells were found to have an affinity for different histochemical stains, including bromophenol blue, iron hematoxylin, Sudan black, Mallory triple trichrome, Crossman's trichrome, Safranin O, and Weigert's stains, as well as lipase and alkaline phosphatase. Endocrine properties of the alarm cells were identified by silver staining and synaptophysin immunostaining. Alarm cells exhibited stemness activities and had a strong immunoaffinity for CD117. They had a proteolytic function, as identified by lysosome-specific staining with acridine orange and strong immunoaffinity for matrix metalloproteinase (MMP-9). They also exhibited proliferatively, reflected by immunological staining by proliferating cell nuclear antigen. In conclusion, alarm cells are unique epidermal cells with multiple functions. They play immunological, and endocrine, roles. They also retain stemness and proliferative properties. |
| تدمد: | 1435-8115 1431-9276 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::b46d00ea6814ad1f35e153c1e3e58beeTest https://doi.org/10.1017/s1431927621012265Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........b46d00ea6814ad1f35e153c1e3e58bee |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14358115 14319276 |
|---|