التفاصيل البيبلوغرافية
| العنوان: |
Apoptotic Effect of Astragalin in Melanoma Skin Cancers via Activation of Caspases and Inhibition of Sry-related HMg-Box Gene 10 |
| المؤلفون: |
Ju-Ha Kim, Sung-Hoon Kim, Jung Hyo Kim, Young-Hwan Kim, Ok Heui You, Hyemin Lee, Jae-Ho Khil, Deok Yong Sim, Eun Ah Shin, Nam-In Baek |
| المصدر: |
Phytotherapy Research. 31:1614-1620 |
| بيانات النشر: |
Wiley, 2017. |
| سنة النشر: |
2017 |
| مصطلحات موضوعية: |
0301 basic medicine, Pharmacology, education.field_of_study, biology, Poly ADP ribose polymerase, Population, Caspase 3, Molecular biology, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, 0302 clinical medicine, Cyclin D1, chemistry, Apoptosis, 030220 oncology & carcinogenesis, biology.protein, Cancer research, Ectopic expression, Astragalin, education, neoplasms, Caspase |
| الوصف: |
Though Astragalin (kaempferol-3-glucoside) contained in Paeonia lactiflora and other plants was known to have anti-oxidant, antiinflammatory, and anti-tumor activity, the anti-tumor mechanism of Astragalin has never been reported in melanomas until now. Thus, in the present study, the underlying apoptotic mechanism of Astragalin isolated from Aceriphyllum rossii was elucidated in A375P and SK-MEL-2 melanoma cells. Astragalin exerted cytotoxicity in A375P and SK-MEL-2 cells in a concentration-dependent manner. Also, Astragalin significantly increased the number of TdT-mediated dUTP nick end labeling positive cells and sub-G1 population as a feature of apoptosis in A375P and SK-MEL-2 cells compared with untreated control. Consistently, western blotting revealed that Astragalin activated caspase 9/3 and Bax, cleaved poly (ADP-ribose) polymerase, and attenuated the expression of cyclin D1, Mcl-1, and Sry-related HMg-Box gene 10 (SOX10) in A375P and SK-MEL-2 cells. Of note, ectopic expression of SOX10 reduced the apoptotic ability of Astragalin to inhibit proliferation, cleave poly (ADP-ribose) polymerase, and caspase 3 in A375P and SK-MEL-2 melanoma cells. Overall, our findings provide evidence that Astragalin induces apoptosis in A375P and SK-MEL-2 melanoma cells via activation of caspase9/3 and inhibition of SOX10 signaling. Copyright © 2017 John Wiley & Sons, Ltd. |
| تدمد: |
0951-418X |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=doi_________::b18ef4d849457be9aa74a62e6c78d7efTest
https://doi.org/10.1002/ptr.5895Test |
| حقوق: |
CLOSED |
| رقم الانضمام: |
edsair.doi...........b18ef4d849457be9aa74a62e6c78d7ef |
| قاعدة البيانات: |
OpenAIRE |
