The purpose of the study is to estimate biomechanical properties such as fracture load and strain in sheep femur bone at 0.2 mm/sec and 2 mm/sec displacement rate. Uniaxial strain gauges (SG) are bonded on a neck-head region of femur bone specimens. The strain is measured by using NI 9236 module and fracture load is measured by using NI9237 module using LabVIEW software. A compressive load is applied on femur head using hydraulic actuator with solenoid operated direction control valve (DCV) during experimentation. The study is intended for compressive loading in-stance configuration at a different displacement rate. A sheep femur bone inclined at 8° in a coronal plane from the distal end and fixed in bone holding fixture. In this study, estimated fracture load of sheep femur bone at a displacement rate of 0.2 mm/sec is 2456.04 ± 324.92 N and strain as 0.00315 ± 0.00107. The tests were conducted at a displacement rate of 2 mm/sec and got the fracture load as 1669.45 ± 269.37 N and strain as 0.0005 ± 0.00025 respectively. It is also found that the fracture load decreases as the displacement rate increases. From experimental testing, it is observed that each one fracture of specimens occurs in a neck-head region. This is often and may be because of the lower mass density in this region of the femur bone. This study also helps to develop material properties for implants and fixation.