French multicentric evaluation of mdr1 gene expression by RT-PCR in leukemia and solid tumours. Standardization of RT-PCR and preliminary comparisons between RT-PCR and immunohistochemistry in solid tumours
| العنوان: | French multicentric evaluation of mdr1 gene expression by RT-PCR in leukemia and solid tumours. Standardization of RT-PCR and preliminary comparisons between RT-PCR and immunohistochemistry in solid tumours |
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| المؤلفون: | Jacques Robert, C Maugard, Pierre Verrelle, Ph Genne, Jean-Pierre Marie, A Clary, M. Raphael, P. Colosetti, JL Merlin, J Charrier, F. Pinguet, L Danel-Moore, B. Richard, Sylvie Chevillard, M. Mousseau, F Calvo, C. Delvincourt, F Finat-Duclos, V Quillien, Pierre Validire, Anne-Marie Faussat, JC Kouyoumdjian, C Bayle, Ph Vielh, P Decrémoux, Lacave R, C Bonnal, Jean Bénard, J. Boutonnat, A Kataki, V. Barbu |
| المصدر: | Leukemia. 11:1095-1106 |
| بيانات النشر: | Springer Science and Business Media LLC, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | Cancer Research, Pathology, medicine.medical_specialty, business.industry, Hematology, Drug resistance, Molecular biology, Reverse transcriptase, law.invention, Multiple drug resistance, Real-time polymerase chain reaction, Oncology, law, Gene expression, Medicine, Immunohistochemistry, Northern blot, business, Polymerase chain reaction |
| الوصف: | Since there is no consensus on the techniques for multidrug resistance (MDR) phenotype evaluation, many discrepancies concerning the importance and frequency of mdr1 gene expression in leukemias and solid tumors are observed in the literature. In order to establish an inter-laboratory consensus in France, a multicenter study was carried out to propose further guidelines for MDR phenotype evaluation. The techniques used by the 38 laboratories participating in the trial were: immunodetection (immunohisto and/or cytochemistry, flow cytometry), functional tests, reverse transcription-polymerase chain reaction (RT-PCR) or Northern blot. We present the results obtained by 19 laboratories concerning the measurement of mdr1 gene expression assessed by RT-PCR or Northern blot in: (1) 19 samples of tumor cells obtained from leukemic patients; (2) six solid tumor samples obtained at surgery; (3) eight cell lines exhibiting variable levels of resistance, and; (4) 10 preparations of RNA and of cDNA obtained from solid tumors. Standardization of the RT-PCR technique and preliminary results comparing RT-PCR with immunohistochemistry in solid tumors are also reported. |
| تدمد: | 1476-5551 0887-6924 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::a7bc5cd14e9db3d44ff3d6f05debaf81Test https://doi.org/10.1038/sj.leu.2400657Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........a7bc5cd14e9db3d44ff3d6f05debaf81 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765551 08876924 |
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