Imbalance between peptidoglycan synthases and hydrolases regulated lysis of Lactobacillus bulgaricus in batch culture
| العنوان: | Imbalance between peptidoglycan synthases and hydrolases regulated lysis of Lactobacillus bulgaricus in batch culture |
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| المؤلفون: | Yifan Wu, Shiwei Chen, Xue Han, Lanwei Zhang, Jialei Sun, Haiyue Niu |
| المصدر: | Archives of Microbiology. 203:4571-4578 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0303 health sciences, Autolysis (biology), Lysis, biology, 030306 microbiology, food and beverages, General Medicine, biology.organism_classification, Biochemistry, Microbiology, Amidase, carbohydrates (lipids), 03 medical and health sciences, Cytosol, chemistry.chemical_compound, chemistry, Lactobacillus, Lactate dehydrogenase, Genetics, Extracellular, Peptidoglycan, Molecular Biology, 030304 developmental biology |
| الوصف: | Lactobacillus bulgaricus is an important starter culture in the dairy industry, cell lysis is negative to the high density of this strain. This work describes the response of peptidoglycan synthases and hydrolases in Lactobacillus bulgaricus sp1.1 when pH decreasing in batch culture. First, the cell lysis was investigated by measuring the cytosolic lactate dehydrogenase released to the fermentation broth, a continuous increase in extracellular lactate dehydrogenase was observed after the lag phase in batch culture. Then, the peptidoglycan hydrolases profile analyzed using the zymogram method showed that eight proteins have the ability of peptidoglycan hydrolysis, three of the eight proteins were considered to contribute lysis of L. bulgaricus sp1.1 according to the changes and extents of peptidoglycan hydrolysis. In silico analysis showed that three putative peptidoglycan hydrolases, including N-acetylmuramyl-L-Ala amidase (protein ID: ALT46642.1), amidase (protein ID: ALT46641.1), and N-acetylmuramidase (protein ID: WP_013439201.1) were compatible with these proteins. Finally, the transcription of the three putative peptidoglycan hydrolases was upregulated in batch culture, in contrast, the expression of four peptidoglycan synthases was downregulated. These observations suggested the imbalance between peptidoglycan synthases and hydrolases involved in the lysis of Lactobacillus bulgaricus sp1.1. |
| تدمد: | 1432-072X 0302-8933 0134-3920 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::8993a080c7bdf40b36bb69a6662a234dTest https://doi.org/10.1007/s00203-021-02433-0Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........8993a080c7bdf40b36bb69a6662a234d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1432072X 03028933 01343920 |
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