التفاصيل البيبلوغرافية
| العنوان: |
CD10, BCL6, and MUM1 expression in diffuse large B-cell lymphoma on FNA samples |
| المؤلفون: |
Valeria Varone, Antonio Vetrani, Gaetano De Rosa, Domenico Memoli, Giuseppe Ciancia, Pio Zeppa, Marco Picardi, Carmine Selleri, Immacolata Cozzolino, Carlo Baldi |
| المصدر: |
Cancer Cytopathology. 124:135-143 |
| بيانات النشر: |
Wiley, 2015. |
| سنة النشر: |
2015 |
| مصطلحات موضوعية: |
0301 basic medicine, Cancer Research, Pathology, medicine.medical_specialty, business.industry, Cancer, Germinal center, medicine.disease, BCL6, Lymphoma, Staining, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Oncology, immune system diseases, hemic and lymphatic diseases, 030220 oncology & carcinogenesis, Medicine, Immunohistochemistry, business, neoplasms, Diffuse large B-cell lymphoma, Immunostaining |
| الوصف: |
BACKGROUND Gene expression profiling has divided diffuse large B-cell lymphoma (DLBCL) into 2 main subgroups: germinal center B (GCB) and non-GCB type. This classification is reproducible by immunohistochemistry using specific antibodies such as CD10, B-cell lymphoma 6 (BCL6), and multiple myeloma oncogene 1 (MUM1). Fine-needle aspiration (FNA) plays an important role in the diagnosis of non-Hodgkin lymphoma, and in some cases FNA may be the only available pathological specimen. The objectives of the current study were to evaluate CD10, BCL6, and MUM1 immunostaining on FNA samples by testing the CD10, BCL6, and MUM1 algorithm on both FNA cell blocks (CB) and conventional smears (CS), evaluating differences in CB and CS immunocytochemical (ICC) performance, and comparing results with histological data. METHODS Thirty-eight consecutive DLBCL cases diagnosed by FNA were studied. Additional passes were used to prepare CB in 22 cases and CS in 16 cases; the corresponding sections and smears were immunostained using CD10, BCL6, and MUM1 in all cases. The data obtained were compared with histological immunostaining in 24 cases. RESULTS ICC was successful in 33 cases (18 CB and 15 CS) and not evaluable in 5 cases (4 CB and 1 CS). The CD10-BCL6-MUM1 algorithm subclassified DLBCL as GCB (9 cases) and non-GCB (24 cases). ICC data were confirmed on histologic staining in 24 cases. CONCLUSIONS CD10, BCL6, and MUM1 ICC staining can be performed on FNA samples. The results herein prove it is reliable both on CB and CS, and is equally effective and comparable to immunohistochemistry data. Cancer (Cancer Cytopathol) 2016;124:135–143. © 2015 American Cancer Society. |
| تدمد: |
1934-662X |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=doi_________::8509fdc8dcbed19aaaa088b5daca5a02Test
https://doi.org/10.1002/cncy.21626Test |
| حقوق: |
OPEN |
| رقم الانضمام: |
edsair.doi...........8509fdc8dcbed19aaaa088b5daca5a02 |
| قاعدة البيانات: |
OpenAIRE |
