FISH and GISH analysis of the genomic relationships amongPanax species
| العنوان: | FISH and GISH analysis of the genomic relationships amongPanax species |
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| المؤلفون: | Jae-Wook Bang, Hae-Woon Choi, Nak-Sul Seong, Dal-Hoe Koo, Kee-Yoeup Paek, Kyong-Hwan Bang |
| المصدر: | Genes & Genomics. 31:99-105 |
| بيانات النشر: | Springer Science and Business Media LLC, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Genetics, biology, food and beverages, Locus (genetics), Ribosomal RNA, biology.organism_classification, complex mixtures, Biochemistry, Genome, Homology (biology), genomic DNA, Ginseng, Botany, Molecular Biology, Gene, American ginseng |
| الوصف: | Ginseng is a well-known medicinal plant that has been used as an anti-aging agent for many years in East Asia. In the genusPanax, only three species,P. ginseng (Oriental ginseng),P. quinquefolius (American ginseng) andP. notoginseng (Chinese ginseng), are currently considered to be important medicinal herbs. Despite the increase in their breeding value, molecular cytogenetic information on the species is not available. To analyze the genomic relationships among thePanax species, FISH (fluorescencein situ hybridization) and GISH (genomicin situ hybridization) techniques were applied. FISH analysis revealed that the 45S and 5S rRNA genes ofP. notoginseng (2n=2x=24) andP. ginseng (2n=4x=48) cluster on a single locus on different chromosomes, whileP. quinquefolius (2n=4x=48),P. japonicus (2n=4x=48), and Korean wild ginseng (2n =4x= 48) had one locus of the 45S rRNA gene and two loci of the 5S rRNA gene, respectively. GISH analysis using genomic DNA as a probe detected strong cross-hybridization of genomes betweenP. ginseng andP. quinquefolius. GISH analysis of other species showed weak or no distinct signals on the chromosomes. Our data revealed thatP. ginseng andP. quinquefolius showed the highest degree of homology, indicating that these species diverged in most recent years. |
| تدمد: | 2092-9293 1976-9571 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::832ce88af3cbded7f7c825958ed86788Test https://doi.org/10.1007/bf03191143Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........832ce88af3cbded7f7c825958ed86788 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20929293 19769571 |
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