التفاصيل البيبلوغرافية
| العنوان: |
In vivodetection of polyomaviruses JCV and SV40 in mesenchymal stem cells from human umbilical cords |
| المؤلفون: |
Alberto Tommasini, Serena Delbue, Manola Comar, Erica Valencic, Elisa Piscianz, Alessandra Tesser, Rossella Del Savio, Pasquale Ferrante, Nunzia Zanotta |
| المصدر: |
Pediatric Blood & Cancer. 61:1347-1349 |
| بيانات النشر: |
Wiley, 2014. |
| سنة النشر: |
2014 |
| مصطلحات موضوعية: |
Stromal cell, viruses, Mesenchymal stem cell, virus diseases, Hematology, Biology, Virology, Umbilical cord, DNA sequencing, chemistry.chemical_compound, medicine.anatomical_structure, Oncology, chemistry, In vivo, Pediatrics, Perinatology and Child Health, medicine, Bone marrow, Merkel cell, DNA |
| الوصف: |
Background Multipotent stromal cells are present in the Wharton's jelly matrix (WJSC) of the umbilical cord and can be used as an allogeneic source of cells to treat immunological disorders. Recently it was demonstrated that adult bone marrow (BM)-derived mesenchimal stromal cells (MSC) are susceptible to infection with viruses showing potential oncogenic properties, such as the polyomavirus JC (JCV). The aim of this study was to investigate the presence of human polyomaviruses (JCV, BK Virus-BKV, SV40, and Merkel cell polyomavirus-MCPyV) in WJSC, and explore the risk of infection. Procedure MSC samples from 35 umbilical cords were investigated by quantitative Real Time PCRs for the presence of DNA sequences of JCV, BKV, SV40, and MCPyV. Results JCV DNA was detected in 1/35 (2.8%) of MSC samples, while SV40 DNA was found in 3/35 (8.6%) of the examined samples. None of the samples showed sequences of BKV and MCPyV. Conclusions The present study demonstrates the in vivo ability of polyomaviruses to infect WJSC. Since the therapeutic approach with the WJSC has high potentiality and a more intensive use can be easily hypothesized, the need to develop consensus guidelines to detect rare viral infections in MSC is pressing. Pediatr Blood Cancer 2014; 61:1347–1349. © 2014 Wiley Periodicals, Inc. |
| تدمد: |
1545-5009 |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=doi_________::7f6288574834bd26008dc5cdc8cb91b7Test
https://doi.org/10.1002/pbc.24943Test |
| حقوق: |
CLOSED |
| رقم الانضمام: |
edsair.doi...........7f6288574834bd26008dc5cdc8cb91b7 |
| قاعدة البيانات: |
OpenAIRE |
