Transcriptome analysis of barley (Hordeum vulgare L.) under waterlogging stress and over-expression of HvADH4 gene confers waterlogging tolerance in transgenic Arabidopsis
التفاصيل البيبلوغرافية
العنوان:
Transcriptome analysis of barley (Hordeum vulgare L.) under waterlogging stress and over-expression of HvADH4 gene confers waterlogging tolerance in transgenic Arabidopsis
Background Waterlogging is one of the major abiotic stresses in barley and greatly reduces grain yield and quality. To explore the mechanism controlling waterlogging tolerance in barley, physiological, anatomical and transcriptional analysis were performed in two contrasting barley varieties viz. Franklin (susceptible) and TX9425 (tolerant). Results Compared to Franklin, more adventitious roots, aerenchymas and higher antioxidant enzymes activities were found in TX9425. 3064 and 5693 differentially expressed genes (DEGs) were identified in TX9425 after 24 h and 72 h waterlogging treatment, respectively, while 2297 and 8462 DEGs were identified in Franklin. The results suggested that TX9425 is less affected by waterlogging stress after 72 h treatment. The DEGs were mainly enriched in energy metabolism, hormone regulation, ROS scavenging, cell wall modifying enzymes and so on. Alcohol dehydrogenase (ADH) plays an important role in response to waterlogging stress. We found that HvADH4 was significantly up-regulated under waterlogging stress in TX9425. The transgenic Arabidopsis overexpressing HvADH4 displayed higher activities of antioxidant enzymes and more tolerant to waterlogging than wild type (WT). Conclusions The current results provide valuable information that will be of great value for the exploration of new candidate genes for barley molecular breeding in waterlogging tolerance.
