The Fragile Fiber1 Kinesin Contributes to Cortical Microtubule-Mediated Trafficking of Cell Wall Components
| العنوان: | The Fragile Fiber1 Kinesin Contributes to Cortical Microtubule-Mediated Trafficking of Cell Wall Components |
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| المؤلفون: | Tobias I. Baskin, Howard Berg, Anindya Ganguly, Daniel D. McClosky, Ram Dixit, Chuanmei Zhu, Ruth Okamoto, Cliff E. Foster, Kristoffer A. Meunier, Charles T. Anderson |
| المصدر: | Plant Physiology. 167:780-792 |
| بيانات النشر: | Oxford University Press (OUP), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Physiology, Vesicle, Plant Science, Golgi apparatus, Biology, Cell biology, Cell wall, symbols.namesake, Microtubule, Genetics, symbols, Kinesin, Secretion, Cortical microtubule, Secondary cell wall |
| الوصف: | The cell wall consists of cellulose microfibrils embedded within a matrix of hemicellulose and pectin. Cellulose microfibrils are synthesized at the plasma membrane, whereas matrix polysaccharides are synthesized in the Golgi apparatus and secreted. The trafficking of vesicles containing cell wall components is thought to depend on actin-myosin. Here, we implicate microtubules in this process through studies of the kinesin-4 family member, Fragile Fiber1 (FRA1). In an fra1-5 knockout mutant, the expansion rate of the inflorescence stem is halved compared with the wild type along with the thickness of both primary and secondary cell walls. Nevertheless, cell walls in fra1-5 have an essentially unaltered composition and ultrastructure. A functional triple green fluorescent protein-tagged FRA1 fusion protein moves processively along cortical microtubules, and its abundance and motile density correlate with growth rate. Motility of FRA1 and cellulose synthase complexes is independent, indicating that FRA1 is not directly involved in cellulose biosynthesis; however, the secretion rate of fucose-alkyne-labeled pectin is greatly decreased in fra1-5, and the mutant has Golgi bodies with fewer cisternae and enlarged vesicles. Based on our results, we propose that FRA1 contributes to cell wall production by transporting Golgi-derived vesicles along cortical microtubules for secretion. |
| تدمد: | 1532-2548 0032-0889 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::6ab5383e748939b52f886bc40b181512Test https://doi.org/10.1104/pp.114.251462Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........6ab5383e748939b52f886bc40b181512 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
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