The role of L-type amino acid transporter 1 (Slc7a5) during in vitro myogenesis
| العنوان: | The role of L-type amino acid transporter 1 (Slc7a5) during in vitro myogenesis |
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| المؤلفون: | Nicolas Collao, Paul Akohene-Mensah, Julian Nallabelli, Emileigh R. Binet, Ali Askarian, Jessica Lloyd, Grace M. Niemiro, Joseph W. Beals, Stephan van Vliet, Rashida Rajgara, Aisha Saleh, Nadine Wiper-Bergeron, Scott A. Paluska, Nicholas A. Burd, Michael De Lisio |
| المصدر: | American Journal of Physiology-Cell Physiology. 323:C595-C605 |
| بيانات النشر: | American Physiological Society, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Physiology, Cell Biology |
| الوصف: | Satellite cells are required for muscle regeneration, remodeling, and repair through their activation, proliferation, and differentiation; however, how dietary factors regulate this process remains poorly understood. The L-type amino acid transporter 1 (LAT1) transports amino acids, such as leucine, into mature myofibers, which then stimulate protein synthesis and anabolic signaling. However, whether LAT1 is expressed on myoblasts and is involved in regulating myogenesis is unknown. The aim of this study was to characterize the expressional and functional relevance of LAT1 during different stages of myogenesis and in response to growth and atrophic conditions in vitro. We determined that LAT1 is expressed by C2C12 and human primary myoblasts, and its gene expression is lower during differentiation ( P < 0.05). Pharmacological inhibition and genetic knockdown of LAT1 impaired myoblast viability, differentiation, and fusion (all P < 0.05). LAT1 protein content in C2C12 myoblasts was not significantly altered in response to different leucine concentrations in cell culture media or in two in vitro atrophy models. However, LAT1 content was decreased in myotubes under atrophic conditions in vitro ( P < 0.05). These findings indicate that LAT1 is stable throughout myogenesis and in response to several in vitro conditions that induce muscle remodeling. Further, amino acid transport through LAT1 is required for normal myogenesis in vitro. |
| تدمد: | 1522-1563 0363-6143 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::698e876e4c07a76c9c8115140185e3a1Test https://doi.org/10.1152/ajpcell.00162.2021Test |
| رقم الانضمام: | edsair.doi...........698e876e4c07a76c9c8115140185e3a1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221563 03636143 |
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