Efficient Production of Nisin A from Low-Value Dairy Side Streams Using a Nonengineered Dairy Lactococcus lactis Strain with Low Lactate Dehydrogenase Activity
| العنوان: | Efficient Production of Nisin A from Low-Value Dairy Side Streams Using a Nonengineered Dairy Lactococcus lactis Strain with Low Lactate Dehydrogenase Activity |
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| المؤلفون: | Robin Dorau, Christian Solem, Jianming Liu, Peter Ruhdal Jensen, Ge Zhao, Jie Zhao |
| المصدر: | Journal of Agricultural and Food Chemistry. 69:2826-2835 |
| بيانات النشر: | American Chemical Society (ACS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, 01 natural sciences, Cofactor, chemistry.chemical_compound, Lactate dehydrogenase, polycyclic compounds, Food science, Nisin, Strain (chemistry), biology, Acetoin, 010401 analytical chemistry, Lactococcus lactis, food and beverages, General Chemistry, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, 0104 chemical sciences, Lactic acid, chemistry, biology.protein, bacteria, lipids (amino acids, peptides, and proteins), Fermentation, General Agricultural and Biological Sciences, 010606 plant biology & botany |
| الوصف: | Nisin is commonly used as a biopreservative in foods. For industrial production, nisin-producing Lactococcus lactis strains are usually grown to high cell densities to achieve the highest possible nisin titer. However, accumulation of lactic acid eventually halts production, even in pH-controlled fermentations. Here, we describe a nisin-producing L. lactis strain Ge001, which was obtained after transferring the nisin gene cluster from L. lactis ATCC 11454, by conjugation, into the natural mutant L. lactis RD1M5, with low lactate dehydrogenase activity. The ability of Ge001 to produce nisin was tested using dairy waste as the fermentation substrate. To accommodate redox cofactor regeneration, respiration conditions were used, and to alleviate oxidative stress and to reduce adsorption of nisin onto the producing cells, we found it to be beneficial to add 1 mM Mn2+ and 100 mM Ca2+, respectively. A high titer of 12 084 IU/mL nisin could be reached, which is comparable to the highest titers reported using expensive, rich media. Summing up, we here present a 100% natural, robust, and sustainable approach for producing food-grade nisin and acetoin from readily available dairy waste. |
| تدمد: | 1520-5118 0021-8561 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::652d15cb4b7f5615c7d912747b094bc0Test https://doi.org/10.1021/acs.jafc.0c07816Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........652d15cb4b7f5615c7d912747b094bc0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205118 00218561 |
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