Preliminary evidence for the existence of specific functional assemblies between enzymes of the β-oxidation pathway and the respiratory chain
| العنوان: | Preliminary evidence for the existence of specific functional assemblies between enzymes of the β-oxidation pathway and the respiratory chain |
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| المؤلفون: | Paul C. Engel, Antony R. Parker |
| المصدر: | Biochemical Journal. 345:429-435 |
| بيانات النشر: | Portland Press Ltd., 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Cytochrome, biology, Respiratory chain, Flavoprotein, Dehydrogenase, Cell Biology, Antimycin A, Biochemistry, Electron-transferring flavoprotein, chemistry.chemical_compound, Sarcosine dehydrogenase, chemistry, Oxidoreductase, biology.protein, Molecular Biology |
| الوصف: | The electron-transferring flavoprotein (ETF) has been detected in two large soluble-protein complexes partially purified from sonicated porcine liver mitochondria. Size-exclusion chromatography and sucrose-density ultracentrifugation suggested molecular masses in the region of 390 to 420 kDa for the two complexes. Activities of medium-chain acyl-CoA dehydrogenase, sarcosine dehydrogenase and ETF:ubiquinone oxidoreductase were also detected. No evidence of oxidative-phosphorylation properties was obtained. Treatment with antimycin A inhibited the activity of both complexes. Pyridine haemochromogens, prepared from the partially purified species, show the presence of cytochrome proteins. The possible composition of these complexes and their relationship to the electron transport chain are discussed. |
| تدمد: | 1470-8728 0264-6021 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::5721ad07cb25ded60b6a89834046e223Test https://doi.org/10.1042/bj3450429Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........5721ad07cb25ded60b6a89834046e223 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14708728 02646021 |
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