Plasmodiophora brassicae in Its Environment: Effects of Temperature and Light on Resting Spore Survival in Soil
| العنوان: | Plasmodiophora brassicae in Its Environment: Effects of Temperature and Light on Resting Spore Survival in Soil |
|---|---|
| المؤلفون: | Kher Zahr, David Feindel, Yalong Yang, H. U. Ahmed, Michael W. Harding, Jie Feng, Alian Sarkes, Qixing Zhou |
| المصدر: | Phytopathology®. 111:1743-1750 |
| بيانات النشر: | Scientific Societies, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, food.ingredient, Resting spore, fungi, Plant Science, Biology, Plasmodiophora brassicae, medicine.disease, Pathogenicity, 01 natural sciences, Spore, Clubroot, 03 medical and health sciences, Horticulture, 030104 developmental biology, food, medicine, Canola, Agronomy and Crop Science, 010606 plant biology & botany |
| الوصف: | Clubroot caused by Plasmodiophora brassicae is an important disease on cruciferous crops worldwide. Management of clubroot is challenging, largely because of the millions of resting spores produced within an infected root that can survive dormant in the soil for many years. This study was conducted to investigate some of the environmental conditions that may affect the survival of resting spores in the soil. Soil samples containing clubroot resting spores (1 × 107 spores/g soil) were stored at various temperatures for 2 years. Additionally, other samples were buried in soil or kept on the soil surface in the field. The content of P. brassicae DNA and the numbers of viable spores in the samples were assessed by quantitative PCR (qPCR) and pathogenicity bioassays, respectively. The results indicated that 4°C, 20°C, and being buried in the soil were more conductive conditions for spore survival than −20°C, 30°C, and at the soil surface. Most (99.99%) of the spores kept on the soil surface were nonviable, suggesting a negative effect of light on spore viability. Additional experiments confirmed the negative effect of ultraviolet light on spore viability because spores receiving 2 and 3 h ultraviolet light exhibited lower disease potential and contained less DNA content than the nontreated control. Finally, this work confirmed that DNA-based quantification methods such as qPCR can be poor predictors of P. brassicae disease potential because of the presence and persistence of DNA from dead spores. |
| تدمد: | 1943-7684 0031-949X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::2dcc7636a73e8aa1fdabdfb0c2fe03fbTest https://doi.org/10.1094/phyto-09-20-0415-rTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........2dcc7636a73e8aa1fdabdfb0c2fe03fb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19437684 0031949X |
|---|