A Photochemical Switch for Controlling Protein-Protein Interactions
| العنوان: | A Photochemical Switch for Controlling Protein-Protein Interactions |
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| المؤلفون: | Sonia K. Pollitt, Peter G. Schultz |
| المصدر: | Angewandte Chemie International Edition. 37:2104-2107 |
| بيانات النشر: | Wiley, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Chemistry, Effector, Mutagenesis, Signal transducing adaptor protein, General Chemistry, GTPase, Photochemistry, Catalysis, Protein–protein interaction, Amino acid, Biochemistry, Side chain, Signal transduction |
| الوصف: | Incorporation of an unnatural amino acid containing a photolabile group in the side chain allows specific interactions between two proteins to be prevented. The photocaged ras protein in which Asp 38 has been substituted by its β-nitrobenzyl ester (Nb) is unable to interact with its effector protein p120-GAP (see drawing below) although it has the same intrinsic GTPase activity. After photocleavage of the Nb group, 50% of the p120-GAP-dependent GTPase activity relative to the wild-type protein is restored. |
| تدمد: | 1521-3773 1433-7851 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::2394ffb950521c154ea50a9e52e2dec3Test https://doi.org/10.1002Test/(sici)1521-3773(19980817)37:15<2104::aid-anie2104>3.0.co;2-z |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........2394ffb950521c154ea50a9e52e2dec3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15213773 14337851 |
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