Comparing the Resynthesis of Brassica napus L. by Interspecific Somatic and Sexual Hybridization. II. Hybrid Morphology and Identifying Organelle Genomes
| العنوان: | Comparing the Resynthesis of Brassica napus L. by Interspecific Somatic and Sexual Hybridization. II. Hybrid Morphology and Identifying Organelle Genomes |
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| المؤلفون: | Pablo Jourdan, Richard H. Ozminkowski |
| المصدر: | Journal of the American Society for Horticultural Science. 119:816-823 |
| بيانات النشر: | American Society for Horticultural Science, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Genetics, education.field_of_study, Somatic cell, Population, Brassica, Horticulture, Biology, biology.organism_classification, Genome, Nuclear DNA, Chloroplast, Botany, Restriction fragment length polymorphism, education, Hybrid |
| الوصف: | Brassica napus (genome aacc), a natural allotetraploid derived from hybridization between B. oleracea L. (genome cc) and B. rapa L. (genome aa), was resynthesized by somatic and sexual hybridization. Seventy-two interspecific somatic (R0) hybrids and 27 sexual (F1) hybrids were produced from the same parent plants. R0 and F1 hybrids displayed morphology that was intermediate to the species parents, but B. rapa characteristics tended to predominate. R0 hybrids with nuclear DNA content equivalent to natural B. napus were uniform for nuclear-encoded traits, whereas allotetraploid F1 hybrids were variable for traits such as morphology, flower color, and seed production. Chloroplast restriction fragment length polymorphisms (RFLPs) showed unequal segregation in the R0 population favoring the chloroplasts of B. rapa; two of the 58 R0 hybrids tested had only the B. oleracea marker and 10 contained markers of both parents. Mitochondrial RFLPs showed a similar bias among the 56 R0 hybrids tested; only four plants showed B. oleracea markers exclusively, and the remaining plants were evenly distributed between having only B. rapa markers or having combinations from both species. In contrast, sexual hybrids displayed only maternal organelle markers. |
| تدمد: | 2327-9788 0003-1062 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::1d461956976fda5669cd7c98cb722de9Test https://doi.org/10.21273/jashs.119.4.816Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........1d461956976fda5669cd7c98cb722de9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23279788 00031062 |
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