Identification of diverse bacterial communities for potential bio-aids capable of troubleshooting for wastewater biological treatment processes
| العنوان: | Identification of diverse bacterial communities for potential bio-aids capable of troubleshooting for wastewater biological treatment processes |
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| المؤلفون: | Y-W Chen, C-Y Huang, H-W Kuo, S-Z Zeng, P-L Lee, C-F Huang, C-R Lin, H-H Chang, C-N Chang, G-Y Li, Y-H Tang, L-Z Chen |
| المصدر: | International Journal of Environmental Science and Technology. 14:743-754 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Environmental Engineering, biology, Phylum, Firmicutes, 030106 microbiology, Bacteroidetes, Computational biology, 010501 environmental sciences, Ribosomal RNA, biology.organism_classification, 01 natural sciences, Microbiology, Actinobacteria, 03 medical and health sciences, Environmental Chemistry, Pyrosequencing, Proteobacteria, General Agricultural and Biological Sciences, Bacteria, 0105 earth and related environmental sciences |
| الوصف: | Three commercial bio-aid samples have shown good troubleshooting capabilities earlier during several biological wastewaters processing, but their microbial contents were still unclear. This study was aimed to assess eubacteria contents for the three bio-aids based on sequence comparisons of 16S ribosomal deoxyribonucleic acid (rDNA). Majority of target 16S rDNA sequences of bacteria within or cultured from bio-aids were obtained via pyrosequencing-based approach, and few sequences were from isolation- or cloning-sequencing approaches. Results showed that a total of 8114, 2327, and 5402 sequences were retrieved from pyrosequencing-based methods for the three bio-aids, respectively, which were affiliated with 100, 80, and 59 bacterial species based on sequence comparison using Ribosome Data Project (RDP) Classifier and Basic Local Alignment Search Tool (BLAST). Target sequences matched Bacillus sp., Enterococcus sp., and Clostridium sp. of Firmicutes phylum were the most abundant for the bio-aids, while some sequences identical to bacteria of phyla Actinobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, and Proteobacteria were also found. Additionally, most of sequences from the isolation- or cloning-sequencing-based methods were not retrieved by the pyrosequencing-based methods. Culture media appeared to potentially select several bacteria species within phyla Firmicutes and/or Proteobacteria, but sequences from the culture-based methods did fulfill some absences for the uncultured method. Based on principal coordinate analysis, bacteria community structures of the three bio-aids were different. This study provided insights into bacteria contents for the three bio-aids as fundamental information in attempts for future selection or formulation of suitable bio-aids for solving various troubles during wastewater biological processing. |
| تدمد: | 1735-2630 1735-1472 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::1b5b4c0ec6d24aaab3da61dcfdeaae70Test https://doi.org/10.1007/s13762-016-1176-zTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........1b5b4c0ec6d24aaab3da61dcfdeaae70 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17352630 17351472 |
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