Long noncoding RNA SYISL regulates myogenesis by interacting with polycomb repressive complex 2
| العنوان: | Long noncoding RNA SYISL regulates myogenesis by interacting with polycomb repressive complex 2 |
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| المؤلفون: | Guoliang Li, Xiaojing Wang, Bo Zuo, Hong Mei Luo, Zai Yan Xu, Shanshan Wang, An Dai Zheng, Rui Zeng, Jianjun Jin, Pan Xia, Wei Lv |
| المصدر: | Proceedings of the National Academy of Sciences. 115 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Myoblast proliferation, Multidisciplinary, Myogenesis, Biology, Long non-coding RNA, Cell biology, 03 medical and health sciences, 030104 developmental biology, Myosin, SYNPO2, Myocyte, C2C12, Myogenin |
| الوصف: | Although many long noncoding RNAs (lncRNAs) have been identified in muscle, their physiological function and regulatory mechanisms remain largely unexplored. In this study, we systematically characterized the expression profiles of lncRNAs during C2C12 myoblast differentiation and identified an intronic lncRNA, SYISL (SYNPO2 intron sense-overlapping lncRNA), that is highly expressed in muscle. Functionally, SYISL promotes myoblast proliferation and fusion but inhibits myogenic differentiation. SYISL knockout in mice results in significantly increased muscle fiber density and muscle mass. Mechanistically, SYISL recruits the enhancer of zeste homolog 2 (EZH2) protein, the core component of polycomb repressive complex 2 (PRC2), to the promoters of the cell-cycle inhibitor gene p21 and muscle-specific genes such as myogenin (MyoG), muscle creatine kinase (MCK), and myosin heavy chain 4 (Myh4), leading to H3K27 trimethylation and epigenetic silencing of target genes. Taken together, our results reveal that SYISL is a repressor of muscle development and plays a vital role in PRC2-mediated myogenesis. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::098657cc4911c3e9447e679ef1ae8cf1Test https://doi.org/10.1073/pnas.1801471115Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........098657cc4911c3e9447e679ef1ae8cf1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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