In Vivo Protein-Protein Interaction Assays: Beyond Proteins
| العنوان: | In Vivo Protein-Protein Interaction Assays: Beyond Proteins |
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| المؤلفون: | Hening Lin, Virginia W. Cornish |
| المصدر: | Angewandte Chemie International Edition. 40:871-875 |
| بيانات النشر: | Wiley, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Reporter gene, Bimolecular fluorescence complementation, Biochemistry, Chemistry, Binding protein, Signal transducing adaptor protein, RNA, General Chemistry, Small molecule, Fusion protein, Catalysis, Protein–protein interaction |
| الوصف: | The scope of cell-based assays is being expanded to allow the detection of interactions between proteins and DNA, RNA, or small molecules. The yeast two-hybrid assay, which normally detects protein-protein interactions, can be modified to detect interactions between ligands (the two dark yellow shapes in the diagram) and receptors (DHFR or GR). A small bridging molecule (made up of the ligands joined together) is used to join two fusion proteins (DHFR-DBD and AD-GR), thereby activating the reporter gene. |
| تدمد: | 1521-3773 1433-7851 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::04c8662b1f0b58985519cfb4f4549993Test https://doi.org/10.1002/1521-3773Test(20010302)40:5<871::aid-anie871>3.0.co;2-s |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........04c8662b1f0b58985519cfb4f4549993 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15213773 14337851 |
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