Mucosal S-IgA Abs is required for complete immunity to Streptococcus pneumoniae (46.6)
| العنوان: | Mucosal S-IgA Abs is required for complete immunity to Streptococcus pneumoniae (46.6) |
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| المؤلفون: | Yoshiko Fukuyama, Janice King, Ryoki Kobayashi, Rebekah Gilbert, Susan Hollingshead, David Briles, Kohtaro Fujihashi |
| المصدر: | The Journal of Immunology. 184:46.6-46.6 |
| بيانات النشر: | The American Association of Immunologists, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | We investigate whether pneumococcal surface protein A (PspA)-specific secretory (S)-IgA antibodies (Abs) induced by nasal vaccine is necessary for the prevention of streptococcal colonization and their clearance. IgA deficient (IgA-/-) and control mice were nasally immunized four times at weekly intervals with PspA plus flt3 ligand plasmid (pFL). One week after the last immunization, PspA-specific S-IgA and IgG Ab as well as Th1- and Th2-type cytokine responses by CD4+ T cells were determined. Further, mice were challenged with Streptococcus pneumoniae (EF3030) via the nasal route. Five days after challenge, nasal washes (NWs) and nasal passages (NPs) were collected and the numbers of bacterial colonies were determined. Mice nasally immunized with PspA plus pFL showed significantly increased levels of Th2 cell-mediated PspA-specific S-IgA and IgG Ab responses in NWs and plasma when compared with naïve mice. Thus, these vaccinated wild type mice showed essentially no bacteria in the nasal cavity. Although IgA-/- mice given nasal PspA plus pFL had significantly high levels of PspA-specific IgG Abs, high numbers of bacteria were detected in NWs and NPs. Further, nasal PspA plus pFL effectively reduced pre-existing S. pneumoniae in the nasal cavity of wild-type, but not IgA-/- mice. These results show that PspA-specific S-IgA Abs play important roles in the prevention of S. pneumoniae colonization and clearance. Supported by NIH grants AG025873 and DE12242. |
| تدمد: | 1550-6606 0022-1767 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::046801777e40e4b07d55f92e6a4a70a6Test https://doi.org/10.4049/jimmunol.184.supp.46.6Test |
| رقم الانضمام: | edsair.doi...........046801777e40e4b07d55f92e6a4a70a6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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