The Expression of P53, MDM2, c-myc, and P14ARF Genes in Newly Diagnosed Acute Lymphoblastic Leukemia Patients
| العنوان: | The Expression of P53, MDM2, c-myc, and P14ARF Genes in Newly Diagnosed Acute Lymphoblastic Leukemia Patients |
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| المؤلفون: | Sina Salari, Mohammad Rafiee, Hamideh Aghaee Nezhad, Arshia Gharehbaghian, Mohammad Hossein Mohammadi, Mehdi Allahbakhshian Farsani |
| المصدر: | Indian Journal of Hematology and Blood Transfusion. 36:277-283 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | medicine.medical_specialty, Hematology, business.industry, Lymphoblast, 030204 cardiovascular system & hematology, Phenotype, Transcriptome, 03 medical and health sciences, 0302 clinical medicine, medicine.anatomical_structure, p14arf, Internal medicine, Gene expression, medicine, Cancer research, Bone marrow, business, Gene, 030215 immunology |
| الوصف: | The treatment response of acute lymphoblastic leukemia (ALL) depends on the percentage of lymphoblasts, cytogenetic aberrations, and altered gene expression. The analysis of the gene expression is applicable for determination of risk stratification and prognosis of cancers. c-MYC, P14ARF, MDM2, and P53 play a vital role in cell survival through a functional network. This study aimed to investigate the expression of these genes, also their correlation with immunophenotypic subtypes of ALL and the percentage of blasts. Real-time PCR was performed for the expression analysis of P53, MDM2, c-MYC, and P14ARF in the bone marrow or peripheral blood samples of 52 ALL patients and 13 normal samples as controls. The morphological analysis and flow cytometry were carried out to examine the phenotypes and percentage of lymphoblasts. The decreased expression levels of P53 and MDM2 were seen in ALL patients compared with control group. In T cell subgroup of ALL the expression of P14ARF gene was more decreased among other subgroups. The expression of MDM2 was decreased in ALL patients who were under the age of 16. Based on our study, the interaction between P53 and MDM2 might be more complex and different from reports published in previous studies. Our findings showed that MDM2 is not negatively correlated with P53, at least in our samples. It can be very effective on the current and future studies to use different techniques for analysis of genome, transcriptome, and proteome in definitive risk stratification and prognosis determination. |
| تدمد: | 0974-0449 0971-4502 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::0125e1678d96b2221d12fd9b349d8109Test https://doi.org/10.1007/s12288-019-01214-6Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........0125e1678d96b2221d12fd9b349d8109 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09740449 09714502 |
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