دورية
Plasmodium berghei:Identification of anmdr-like Gene Associated with Drug Resistance
| العنوان: | Plasmodium berghei:Identification of anmdr-like Gene Associated with Drug Resistance |
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| المؤلفون: | Gervais, Gary W, Trujillo, Kathy, Robinson, Brian L, Peters, Wallace, Serrano, Adelfa E |
| المصدر: | Experimental Parasitology; January 1999, Vol. 91 Issue: 1 p86-92, 7p |
| مستخلص: | Gervais, G. W., Trujillo, K., Robinson, B. L., Peters, W., and Serrano, A. E. 1999.Plasmodium berghei:Identification of anmdr-like gene associated with drug resistance.Experimental Parasitology91,86–92. Amplification, mutations, or overexpression of thepfmdr1gene have been associated with multiple drug resistance in some strains ofPlasmodium falciparum.In order to better understand this potential mechanism of drug resistance, we are currently investigating putativemdrhomologuesin vivoin the rodent malariaPlasmodium berghei.We have identified and partially sequenced a gene that is amplified in a MFQ-resistant (MFQr) line. Using degenerate primers, a 579-bp fragment was amplified by PCR usingP. bergheigenomic DNA as template. The predicted amino acid sequence shares 66% identity with the previously reportedpfmdr1gene product (Pgh1) ofP. falciparum.Southern blots and slot blots of genomic DNA suggest that this gene is amplified two- to threefold in a MFQrline (N/1100), as has been previously reported in some MFQrstrains ofP. falciparum.TheP. bergheigene was mapped to chromosome 12 in all of the lines analyzed. Furthermore, the cloned PCR product also hybridizes to chromosome 5 of the MFQrstrain. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00144894 10902449 |
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| DOI: | 10.1006/expr.1999.4344 |