دورية
A yeast based assay establishes the pathogenicity of novel missense ACTA2variants associated with aortic aneurysms
| العنوان: | A yeast based assay establishes the pathogenicity of novel missense ACTA2variants associated with aortic aneurysms |
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| المؤلفون: | Calderan, Cristina, Sorrentino, Ugo, Persano, Luca, Trevisson, Eva, Sartori, Geppo, Salviati, Leonardo, Desbats, Maria Andrea |
| المصدر: | European Journal of Human Genetics: EJHG; 20240101, Issue: Preprints p1-9, 9p |
| مستخلص: | The ACTA2gene codes for alpha-smooth muscle actin, a critical component of the contractile apparatus of the vascular smooth muscle cells. Autosomal dominant variants in the ACTA2gene have been associated to familial non-syndromic thoracic aortic aneurysm/dissection (TAAD). They are thought to act through a dominant-negative mechanism. These variants display incomplete penetrance and variable expressivity, complicating the validation of ACTA2variants pathogenicity by family segregation studies. In this study, we developed a yeast based assay to test putative TAAD-associated ACTA2variants. We identified five new heterozygous ACTA2missense variants in TAAD patients through next generation sequencing. We decided to test their pathogenicity in Saccharomyces cerevisiae, since yeast actin is very similar to human alpha-smooth muscle actin, and the residues at which the TAAD-associated variants occur in ACTA2 are well conserved. A wild type yeast strain was transformed with a vector expressing the different mutant alleles, to model the heterozygous condition of patients. Then, we evaluated yeast growth by spot test and cytoskeletal and mitochondrial morphology by fluorescence microscopy. We found that mutant yeast strains displayed only mild growth defects but a significant increase in the percentage of cells with abnormal mitochondrial distribution and abnormal organization of the actin cytoskeleton compared to controls. All variants appeared to interfere with the activity of wild type actin in yeast, suggesting a dominant-negative pathogenic mechanism. Our results demonstrate the utility of using the yeast actin model system to validate the pathogenicity of TAAD-associated ACTA2variants. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 10184813 14765438 |
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| DOI: | 10.1038/s41431-024-01591-1 |