دورية
Staphylococcusnasal colonization in three species of non-human primates
| العنوان: | Staphylococcusnasal colonization in three species of non-human primates |
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| المؤلفون: | da Silva, Juliana Georg, Culuchi, Glenda, Pestana, Cristiane Pinheiro, da Silva Junior, Haroldo Cid, Saraiva, Felipe Betoni, Kugelmeier, Tatiana, Rouede, Daniel, Pinto, Ana Cristina Araújo, Pissinati, Thalita, D’Alincourt Assef, Ana Paula, Rocha-de-Souza, Claudio Marcos, e Oliveira, Thamirys Rachel Tavares, Senna, José Procópio M |
| المصدر: | Brazilian Journal of Microbiology; 20230101, Issue: Preprints p1-8, 8p |
| مستخلص: | Bacterial nasal colonization is common in many mammals and Staphylococcusrepresents the main pathogen isolated. Staphylococcusnasal carriage in humans constitutes a risk factor for Staphylococcusinfections pointing out the need for animal experimentation for nasal colonization studies, especially for vaccine development. A limitation in addressing this hypothesis has been a lack of appropriate animal model. Murine models do not mimic human nasal colonization studies. Non-human primates (NHP) remain the best classical models for nasal colonization studies. In this study, we analyzed nasal colonization between two species of Old World monkeys (cynomolgus and rhesus) and a New World monkey (squirrel monkey) from breeding colony at Fiocruz (Brazil). Sixty male and female NHP with the average age of 1–21 years old, comprising twenty animals of each species, were analyzed. Nine different Staphylococcus species (S. aureus, S. cohnii, S. saprophyticus, S. haemolyticus, S. xylosus, S. warneri, S. nepalensis, S. simiae, and S. kloosi) were identified by MALDI-TOF and 16S rRNA gene sequence analyses. Antibiotic resistance was not detected among the isolated bacterial population. S. aureuswas the main isolate (19 strains), present in all species, predominant in cynomolgus monkeys (9/20) and squirrel monkeys (7/20). spatyping was used to examine the clonal structure and genetic profile of Staphylococcus aureusisolates. Eight (8) spa types were identified among the S. aureusstrains. A major cluster was identified, corresponding to a new spatype t20455, and no spatypes found in this study were seen before in Brazil. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 15178382 16784405 |
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| DOI: | 10.1007/s42770-023-00959-7 |