دورية
P-selectin glycoprotein ligand-1 supports rolling on E- and P-selectin in vivo
| العنوان: | P-selectin glycoprotein ligand-1 supports rolling on E- and P-selectin in vivo |
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| المؤلفون: | Norman, Keith E., Katopodis, Andreas G., Thoma, Gebhard, Kolbinger, Frank, Hicks, Anne E., Cotter, Matthew J., Pockley, A. Graham, Hellewell, Paul G. |
| المصدر: | Blood; November 2000, Vol. 96 Issue: 10 p3585-3591, 7p |
| مستخلص: | Selectin-dependent rolling is the earliest observable event in the recruitment of leukocytes to inflamed tissues. Several glycoproteins decorated with sialic acid, fucose, and/or sulfate have been shown to bind the selectins. The best-characterized selectin ligand is P-selectin glycoprotein-1 (PSGL-1) that supports P-selectin– dependent rolling in vitro and in vivo. In vitro studies have suggested that PSGL-1 may also be a ligand for E- and L-selectins. To study the in vivo function of PSGL-1, without the influence of other leukocyte proteins, the authors observed the interaction of PSGL-1–coated microspheres in mouse venules stimulated to express P- and/or E-selectin. Microspheres coated with functional recombinant PSGL-1 rolled in surgically stimulated and tumor necrosis factor alpha (TNFa)-stimulated mouse venules. P-selectin deficiency or inhibition abolished microsphere rolling in surgically and TNFa-stimulated venules, whereas E-selectin deficiency or inhibition increased microsphere rolling velocity in TNFa-stimulated venules. The results suggest that P-selectin–PSGL-1 interaction alone is sufficient to mediate rolling in vivo and that E-selectin–PSGL-1 interaction supports slow rolling. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00064971 15280020 |
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| DOI: | 10.1182/blood.V96.10.3585 |