دورية
Dichlorodiphenylchloroethylene Elevates Cytosolic Calcium Concentrations and Oscillations in Primary Cultures of Human Granulosa-Lutein Cells1
| العنوان: | Dichlorodiphenylchloroethylene Elevates Cytosolic Calcium Concentrations and Oscillations in Primary Cultures of Human Granulosa-Lutein Cells1 |
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| المؤلفون: | Younglai, E.V., Kwan, T. K., Kwan, C.-Y., Lobb, D.K., Foster, W.G. |
| المصدر: | Biology of Reproduction; June 2004, Vol. 70 Issue: 6 p1693-1700, 8p |
| مستخلص: | 1,1-Dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE), a metabolite of DDT (1,1-dichlorodiphenyltrichloroethane), is a persistent hormonally active environmental toxicant that has been found in human serum and follicular fluid. The objective of this study was to determine whether DDE can alter free calcium ion concentrations in the cytosol ([Ca2+]cyt) of human granulosa cells. Changes in [Ca2+]cytin single cells loaded with Fura-2 were studied using a dynamic digital Ca2+imaging system. At a concentration of 100 ng/ml, DDE stimulated small elevations of [Ca2+]cytaccompanied by Ca2+oscillations. At 1 μg DDE/ml, there was a biphasic Ca2+response with marked elevations of [Ca2+]cytover time. In Ca2+-free medium, cells showed an initial small elevation of [Ca2+]cyt, which was magnified after addition of Ca2+to the medium. Washing the cells after DDE treatment failed to remove the elevated [Ca2+]cytand oscillations, both of which were eliminated by addition of EGTA. ATP also induced [Ca2+]cytelevations and oscillations, and these effects were potentiated when DDE was added. FSH induced transient [Ca2+]cytelevations, whereas hCG caused a prolonged elevation and marked oscillations in [Ca2+]cyt. These results suggest that DDE at concentrations normally found in human tissues induces elevations in [Ca2+]cytin granulosa-lutein cells. Our data therefore highlight a novel mechanism through which DDE can alter endocrine homeostasis and possibly act as an endocrine toxicant. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00063363 15297268 |
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| DOI: | 10.1095/biolreprod.103.026187 |