دورية
Tsp57: A Novel Gene Induced During a Specific Stage of Spermatogenesis
| العنوان: | Tsp57: A Novel Gene Induced During a Specific Stage of Spermatogenesis |
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| المؤلفون: | Kim, Yong-Sik, Nakanishi, Gen, Oudes, Asa J., Kim, Kwan Hee, Wang, Hang, Kilpatrick, Daniel L., Jetten, Anton M. |
| المصدر: | Biology of Reproduction; January 2004, Vol. 70 Issue: 1 p106-113, 8p |
| مستخلص: | Recently, we described the identification of a novel protein, nuclear receptor-associated protein 80 (RAP80), which is highly expressed in spermatocytes and appears to have a role in regulating gene expression. To identify proteins interacting with this protein, we performed yeast two-hybrid screening using full-length RAP80 as bait. This screen identified one in-frame clone encoding a novel testis-specific protein (Tsp), referred to as Tsp57. Tsp57encodes a basic protein with a mass of 56.8 kDa. The amino acid sequence of Tsp57 is highly conserved (87%) between mouse and human. The mouse and human Tsp57genes map to chromosomes 9A1 and 11q21, respectively. Northern blot analysis showed that the expression of Tsp57mRNA was highly restricted to the testis and temporally regulated during testicular development. Tsp57mRNA was greatly induced between Day 21 and Day 25 of postnatal testicular development. In situ hybridization analysis demonstrated that the hybridization signal for Tsp57mRNA was strongest in sections of seminiferous tubules at stages VI–VIII of spermatogenesis, consistent with the conclusion that Tsp57is most highly expressed in round spermatids. Study of Tsp57expression in several purified subpopulations of spermatogenic cells confirmed maximum levels of expression in round spermatids. Consistently, Tsp57expression was absent in testes from vitamin A-deficient mice, which do not have any round spermatids, and was reduced in RARα null mice, which have lowered numbers of round spermatids in their testes. These results indicate the possibility that Tsp57 protein plays a role in the postmeiotic phase of germ cell differentiation. Tsp57 contains two putative nuclear localization signals: NLS1 and NLS2. Examination of the cellular localization showed that the green fluorescent protein-Tsp57 fusion protein localized to both cytoplasm and nucleus. After deletion of NLS1 but not NLS2, Tsp57 localized solely to the cytoplasm, indicating a role for NLS1 in the nuclear localization of Tsp57. The localization suggests a nuclear function for Tsp57. Pull-down analysis demonstrated that Tsp57 and RAP80 form a complex in intact cells. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00063363 15297268 |
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| DOI: | 10.1095/biolreprod.103.018465 |