دورية
asunderIs a Critical Regulator of Dynein–Dynactin Localization during DrosophilaSpermatogenesis
| العنوان: | asunderIs a Critical Regulator of Dynein–Dynactin Localization during DrosophilaSpermatogenesis |
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| المؤلفون: | Anderson, Michael A., Jodoin, Jeanne N., Lee, Ethan, Hales, Karen G., Hays, Thomas S., Lee, Laura A. |
| المصدر: | Molecular Biology of the Cell; June 2009, Vol. 20 Issue: 11 p2709-2721, 13p |
| مستخلص: | Spermatogenesis uses mitotic and meiotic cell cycles coordinated with growth and differentiation programs to generate functional sperm. Our analysis of a Drosophilamutant has revealed that asunder(asun), which encodes a conserved protein, is an essential regulator of spermatogenesis. asunspermatocytes arrest during prophase of meiosis I. Strikingly, arrested spermatocytes contain free centrosomes that fail to stably associate with the nucleus. Spermatocytes that overcome arrest exhibit severe defects in meiotic spindle assembly, chromosome segregation, and cytokinesis. Furthermore, the centriole-derived basal body is detached from the nucleus in asunpostmeiotic spermatids, resulting in abnormalities later in spermatogenesis. We find that asunspermatocytes and spermatids exhibit drastic reduction of perinuclear dynein–dynactin, a microtubule motor complex. We propose a model in which asuncoordinates spermatogenesis by promoting dynein–dynactin recruitment to the nuclear surface, a poorly understood process required for nucleus–centrosome coupling at M phase entry and fidelity of meiotic divisions. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 10591524 19394586 |
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| DOI: | 10.1091/mbc.e08-12-1165 |