دورية
In Vitro Analysis of d-Lactyl-CoA-Polymerizing Polyhydroxyalkanoate Synthase in Polylactate and Poly(lactate-co-3-hydroxybutyrate) Syntheses
| العنوان: | In Vitro Analysis of d-Lactyl-CoA-Polymerizing Polyhydroxyalkanoate Synthase in Polylactate and Poly(lactate-co-3-hydroxybutyrate) Syntheses |
|---|---|
| المؤلفون: | Matsumoto, Ken’ichiro, Iijima, Midori, Hori, Chiaki, Utsunomia, Camila, Ooi, Toshihiko, Taguchi, Seiichi |
| المصدر: | Biomacromolecules; July 2018, Vol. 19 Issue: 7 p2889-2895, 7p |
| مستخلص: | Engineered d-lactyl-coenzyme A (LA-CoA)-polymerizing polyhydroxyalkanoate synthase (PhaC1PsSTQK) efficiently produces poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB]) copolymer in recombinant Escherichia coli, while synthesizing tiny amounts of poly(lactate) (PLA)-like polymers in recombinant Corynebacterium glutamicum. To elucidate the mechanisms underlying the interesting phenomena, in vitroanalysis of PhaC1PsSTQK was performed using homo- and copolymerization conditions of LA-CoA and 3-hydroxybutyryl-CoA. PhaC1PsSTQK polymerized LA-CoA as a sole substrate. However, the extension of PLA chains completely stalled at a molecular weight of ∼3000, presumably due to the low mobility of the generated polymer. The copolymerization of these substrates only proceeded with a low concentration of LA-CoA. In fact, the intracellular LA-CoA concentration in P(LA-co-3HB)-producing E. coliwas below the detection limit, while that in C. glutamicumwas as high as acetyl-CoA levels. Therefore, it was concluded that the mobility of polymerized products and LA-CoA concentration are dominant factors characterizing PLA and P(LA-co-3HB) biosynthetic systems. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 15257797 15264602 |
|---|---|
| DOI: | 10.1021/acs.biomac.8b00454 |