دورية
Dynamic Changes of Intracellular Monomer Levels Regulate Block Sequence of Polyhydroxyalkanoates in Engineered Escherichia coli
| العنوان: | Dynamic Changes of Intracellular Monomer Levels Regulate Block Sequence of Polyhydroxyalkanoates in Engineered Escherichia coli |
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| المؤلفون: | Matsumoto, Ken’ichiro, Hori, Chiaki, Fujii, Ryunosuke, Takaya, Masahiro, Ooba, Takashi, Ooi, Toshihiko, Isono, Takuya, Satoh, Toshifumi, Taguchi, Seiichi |
| المصدر: | Biomacromolecules; 20240101, Issue: Preprints |
| مستخلص: | Biological polymer synthetic systems, which utilize no template molecules, normally synthesize random copolymers. We report an exception, a synthesis of block polyhydroxyalkanoates (PHAs) in an engineered Escherichia coli. Using an engineered PHA synthase, block copolymers poly[(R)-2-hydroxybutyrate(2HB)-b-(R)-3-hydroxybutyrate(3HB)] were produced in E. coli. The covalent linkage between P(2HB) and P(3HB) segments was verified with solvent fractionation and microphase separation. Notably, the block sequence was generated under the simultaneous consumption of two monomer precursors, indicating the existence of a rapid monomer switching mechanism during polymerization. Based on in vivometabolic intermediate analysis and the relevant in vitroenzymatic activities, we propose a model in which the rapid intracellular 3HB-CoA fluctuation during polymer synthesis is a major factor in generating block sequences. The dynamic change of intracellular monomer levels is a novel regulatory principle of monomer sequences of biopolymers. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 15257797 15264602 |
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| DOI: | 10.1021/acs.biomac.7b01768 |