دورية
Encapsulation of an Enzyme Cascade within the Bacteriophage P22 Virus-Like Particle
| العنوان: | Encapsulation of an Enzyme Cascade within the Bacteriophage P22 Virus-Like Particle |
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| المؤلفون: | Patterson, Dustin P., Schwarz, Benjamin, Waters, Ryan S., Gedeon, Tomas, Douglas, Trevor |
| المصدر: | ACS Chemical Biology; February 2014, Vol. 9 Issue: 2 p359-365, 7p |
| مستخلص: | Developing methods for investigating coupled enzyme systems under conditions that mimic the cellular environment remains a significant challenge. Here we describe a biomimetic approach for constructing densely packed and confined multienzyme systems through the co-encapsulation of 2 and 3 enzymes within a virus-like particle (VLP) that perform a coupled cascade of reactions, creating a synthetic metabolon. Enzymes are efficiently encapsulated in vivowith known stoichiometries, and the kinetic parameters of the individual and coupled activities are characterized. From the results we develop and validate a mathematical model for predicting the expected kinetics for coupled reactions under co-localized conditions. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 15548929 15548937 |
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| DOI: | 10.1021/cb4006529 |