التفاصيل البيبلوغرافية
| العنوان: |
Changes in cargoes of platelet derived extracellular vesicles heterogeneous subpopulations induced by PM0.1——Undisclosed cardiovascular injury communication mechanism. |
| المؤلفون: |
Mao, Zhen, Wu, Yingting, Kong, Ling, Zhou, Lihong, Zhang, Xiaodan, Geng, Aobo, Cai, Jin, Yang, Hong, Peili, Huang |
| المصدر: |
Environmental Pollution; May2024, Vol. 348, pN.PAG-N.PAG, 1p |
| مصطلحات موضوعية: |
EXTRACELLULAR vesicles, FIELD-flow fractionation, MEMBRANE fusion, PLATELET-rich plasma, UMBILICAL veins |
| مستخلص: |
Epidemiological evidence has indicated a closely link between PM 0.1 exposure and the incidence rate of cardiovascular diseases. This study explores the underlying communication roles of platelet-derived extracellular vesicles (PEVs) heterogeneous subpopulations in cardiovascular injury. PEVs and PMEVs which were extracted from platelet-rich plasma (PRP) un-exposure or exposure to PM 0.1 by TIM4 affinity beads. By optimizing separation conditions, replacing pipelines, and resetting injection procedures, Asymmetric flow field-flow fractionation (AF4) was employed to separate, purify, characterize, and enrich PEVs and PMEVs heterogeneous subpopulations (small PEVs, PEVs-S/PMEVs-S: <100 nm; medium PEVs, PEVs-M/PMEVs-M: 100–200 nm; and large PEVs, PEVs-L/PMEVs-L: >200 nm). The results showed that the cargoes of PMEVs heterogeneous subpopulations which were released by PRP stimulated by PM 0.1 were changed obviously. Moreover, compared with PEVs, PMEVs can lead to a decrease in the survival rate of Human Umbilical Vein Endothelial Cells (HUVECs). In PMEVs-S subpopulations, the alterations of lipids associated with membrane fusion and cell signaling transport (such as PC, Cer), as well as miRNAs related to inflammation, angiogenesis, and migration (miR-223, miR-22, miR-126, and miR-150), are similar to those in PMEVs-M subpopulations but distinct from PMEVs-L subpopulations. This study revealed the diverse communication mechanisms underlying PM 0.1 -induced cardiovascular injury, thereby offering potential avenues for the development of new biomarkers and therapeutic targets. [Display omitted] • AF4 is a strong tool to isolate and enrich EVs heterogeneous subpopulations. • Exposure to PM 0.1 can cause changes in cargo of PEVs heterogeneous subpopulations. • Lipid and miRNA changes in PEVs are vital for PM 0.1 -induced cardiovascular injury. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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