التفاصيل البيبلوغرافية
| العنوان: |
Genes from Carboxypeptidase A, glutathione S-transferase, and cytochrome b families were found involved in lead transport in insect Musca domestica. |
| المؤلفون: |
Wang, Wanqiang, Tang, Wenzheng, Wang, Xiaoping, Liu, Wen, Zhu, Fen |
| المصدر: |
Ecotoxicology & Environmental Safety; Jan2022, Vol. 230, pN.PAG-N.PAG, 1p |
| مصطلحات موضوعية: |
CARBOXYPEPTIDASES, CYTOCHROME b, HOUSEFLY, GLUTATHIONE transferase, GLUTATHIONE, INSECTS |
| مستخلص: |
Lead (Pb) is a typical toxic contamination source all over the world. In this research, larvae of the housefly (Musca domestica) were fed a Pb-contaminated diet at different Pb doses of 0, 20 and 5000 mg/kg. RNA sequencing was used to identify genes that were differentially expressed in relation to lead transport or detoxification. RNA interference (RNAi) was carried on 12 candidate genes. The results showed that three luminal pH regions of mid-gut were at pH values of 6.33, 3.10, and 7.80. With increasing Pb concentration, the pH of the middle mid-gut decreased by one unit. The expression levels of carboxypeptidase A (CPA1), glutathione S-transferase (GST), and cytochrome b (Cyt b) were linked to Pb treatments, particularly high Pb concentration of 5000 mg/kg. RNAi-mediated down expression of CPA1 , GST2 , and CYTb-c1 resulted in low Pb accumulation in the larvae of 5000 mg/kg Pb group. These proteins played key roles in Pb transport and detoxification in M. domestica larvae. • Three luminal pH regions were found in mid-gut of M. domestica larvae. • Middle of mid-gut pH appeared a decrease with increase of Pb concentration. • RNAi of CPA1 , and GST2 displayed Pb accumulation drop of 30%, and 30.7%. • CPA , GST , and CYT b are was involved in Pb transportation of M. domestica. [ABSTRACT FROM AUTHOR] |
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