التفاصيل البيبلوغرافية
| العنوان: |
Generation and characterization of three induced pluripotent stem cell lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a 7-year-old healthy Caucasian male. |
| المؤلفون: |
Ge, Ning, Liu, Min, Zhu, Xiaoran, Krawczyk, Janusz, McInerney, Veronica, Shen, Sanbing, O'Brien, Timothy, Prendiville, Terence |
| المصدر: |
Stem Cell Research; May2021, Vol. 53, pN.PAG-N.PAG, 1p |
| مستخلص: |
We report the generation of three human induced pluripotent stem cell (hiPSC) lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a healthy 7-year-old boy using non-integrational Sendai re-programming method expressing OCT4, SOX2, KLF4 and C-MYC. Stem cell characterization was confirmed through morphology, immunofluorescence staining and RT-qPCR. Differentiation potential in vitro was demonstrated to all three germ layers with STR lineage verification and normal molecular karyotyping through the process of re-programming. [ABSTRACT FROM AUTHOR] |
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