التفاصيل البيبلوغرافية
| العنوان: |
Biomedical applications of nanoflares: Targeted intracellular fluorescence probes. |
| المؤلفون: |
Chenab, Karim Khanmohammadi, Eivazzadeh-Keihan, Reza, Maleki, Ali, Pashazadeh-Panahi, Paria, Hamblin, Michael R, Mokhtarzadeh, Ahad |
| المصدر: |
Nanomedicine: Nanotechnology, Biology & Medicine; Apr2019, Vol. 17, p342-358, 17p |
| مصطلحات موضوعية: |
NUCLEIC acids, SINGLE-stranded DNA, GOLD nanoparticles, OLIGONUCLEOTIDES, FLUORESCENCE, NUCLEIC acid hybridization |
| مستخلص: |
Nanoflares are intracellular probes consisting of oligonucleotides immobilized on various nanoparticles that can recognize intracellular nucleic acids or other analytes, thus releasing a fluorescent reporter dye. Single-stranded DNA (ssDNA) complementary to mRNA for a target gene is constructed containing a 3′-thiol for binding to gold nanoparticles. The ssDNA "recognition sequence" is prehybridized to a shorter DNA complement containing a fluorescent dye that is quenched. The functionalized gold nanoparticles are easily taken up into cells. When the ssDNA recognizes its complementary target, the fluorescent dye is released inside the cells. Different intracellular targets can be detected by nanoflares, such as mRNAs coding for genes over-expressed in cancer (epithelial-mesenchymal transition, oncogenes, thymidine kinase, telomerase, etc.), intracellular levels of ATP, pH values and inorganic ions can also be measured. Advantages include high transfection efficiency, enzymatic stability, good optical properties, biocompatibility, high selectivity and specificity. Multiplexed assays and FRET-based systems have been designed. Biomedical applications of nanoflares in biosensing. Nanoflares consist of oligonucleotides attached to gold nanoparticles that can release a fluorescent dye upon binding to their target. The dye excitation is wavelength matched to the plasmon resonance frequency of the nanoparticles. They are able to detect intracellular analytes such as mRNAs and ATP with high sensitivity, and can also respond to pH and metallic ions. Unlabelled Image [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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