التفاصيل البيبلوغرافية
| العنوان: |
Mapping and genotypic analysis of the NK-lysin gene in chicken. |
| المؤلفون: |
Mi Ok Lee, Yang, Ence, Morisson, Mireille, Vignal, Alain, Yong-Zhen Huang, Cheng, Hans H., Muir, William M., Lamont, Susan J., Hyun Soon Lillehoj, Sung Hyen Lee, Womack, James E. |
| المصدر: |
Genetics Selection Evolution; Jul2014, Vol. 46, p1-7, 7p |
| مصطلحات موضوعية: |
ANIMAL genetics, ANTI-infective agents, CHICKENS, GENETIC polymorphisms, NUCLEOTIDE sequence, LYSINS |
| مستخلص: |
Background: Antimicrobial peptides (AMP) are important elements of the first line of defence against pathogens in animals. NK-lysin is a cationic AMP that plays a critical role in innate immunity. The chicken NK-lysin gene has been cloned and its antimicrobial and anticancer activity has been described but its location in the chicken genome remains unknown. Here, we mapped the NK-lysin gene and examined the distribution of a functionally significant single nucleotide polymorphism (SNP) among different chicken inbred lines and heritage breeds. Results: A 6000 rad radiation hybrid panel (ChickRH6) was used to map the NK-lysin gene to the distal end of chromosome 22. Two additional genes, the adipocyte enhancer-binding protein 1-like gene (AEBP1) and the DNA polymerase delta subunit 2-like (POLD2) gene, are located in the same NW_003779909 contig as NK-lysin, and were thus indirectly mapped to chromosome 22 as well. Previously, we reported a functionally significant SNP at position 271 of the NK-lysin coding sequence in two different chicken breeds. Here, we examined this SNP and found that the A allele appears to be more common than the G allele in these heritage breeds and inbred lines. Conclusions: The chicken NK-lysin gene mapped to the distal end of chromosome 22. Two additional genes, AEBP1 and POLD2, were indirectly mapped to chromosome 22 also. SNP analyses revealed that the A allele, which encodes a peptide with a higher antimicrobial activity, is more common than the G allele in our tested inbred lines and heritage breeds. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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