التفاصيل البيبلوغرافية
| العنوان: |
Mice deficient in heparanase exhibit impaired dendritic cell migration and reduced airway inflammation. |
| المؤلفون: |
Poon, Ivan K. H., Goodall, Katharine J., Phipps, Simon, Chow, Jenny D. Y., Pagler, Eloisa B., Andrews, Daniel M., Conlan, Carly L., Ryan, Gemma F., White, Julie A., Wong, Michael K. L., Horan, Catherine, Matthaei, Klaus I., Smyth, Mark J., Hulett, Mark D. |
| المصدر: |
European Journal of Immunology; Apr2014, Vol. 44 Issue 4, p1016-1030, 15p |
| مستخلص: |
Heparanase is a β- d-endoglucuronidase that cleaves heparan sulphate, a key component of the ECM and basement membrane. The remodelling of the ECM by heparanase has been proposed to regulate both normal physiological and pathological processes, including wound healing, inflammation, tumour angiogenesis and cell migration. Heparanase is also known to exhibit non-enzymatic functions by regulating cell adhesion, cell signalling and differentiation. In this study, constitutive heparanase-deficient (Hpse−/−) mice were generated on a C57BL/6 background using the Cre/loxP recombination system, with a complete lack of heparanase mRNA, protein and activity. Although heparanase has been implicated in embryogenesis and development, Hpse−/− mice are anatomically normal and fertile. Interestingly, consistent with the suggested function of heparanase in cell migration, the trafficking of dendritic cells from the skin to the draining lymph nodes was markedly reduced in Hpse−/− mice. Furthermore, the ability of Hpse−/− mice to generate an allergic inflammatory response in the airways, a process that requires dendritic cell migration, was also impaired. These findings establish an important role for heparanase in immunity and identify the enzyme as a potential target for regulation of an immune response. [ABSTRACT FROM AUTHOR] |
|
Copyright of European Journal of Immunology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
