التفاصيل البيبلوغرافية
| العنوان: |
Evidence of the transient nature of the Th17 phenotype of CD4+CD161+ T cells in the synovial fluid of patients with juvenile idiopathic arthritis. |
| المؤلفون: |
Cosmi, Lorenzo, Cimaz, Rolando, Maggi, Laura, Santarlasci, Veronica, Capone, Manuela, Borriello, Francesco, Frosali, Francesca, Querci, Valentina, Simonini, Gabriele, Barra, Giusi, Piccinni, Marie Pierre, Liotta, Francesco, De Palma, Raffaele, Maggi, Enrico, Romagnani, Sergio, Annunziato, Francesco |
| المصدر: |
Arthritis & Rheumatism; Aug2011, Vol. 63 Issue 8, p2504-2515, 12p |
| مصطلحات موضوعية: |
T cells, BLOOD testing, STATISTICAL correlation, ENZYME-linked immunosorbent assay, INTERLEUKINS, RESEARCH funding, JUVENILE idiopathic arthritis, SYNOVIAL fluid, T-test (Statistics), EQUIPMENT & supplies, PHYSIOLOGY |
| مصطلحات جغرافية: |
ITALY |
| مستخلص: |
Objective To investigate the phenotype and function of CD4+ T cells in synovial fluid (SF) from the affected joints of children with oligoarticular-onset juvenile idiopathic arthritis (JIA), and to establish a possible link with disease activity. Methods CD4+ T cells were obtained from the peripheral blood (PB) and SF of 23 children with oligoarticular-onset JIA, as well as from the PB of 15 healthy children. The cells were analyzed for the expression of CXCR3, CCR6, and CD161 and for the production of interferon-γ and interleukin-17A (IL-17A). Spectratyping and clonotype analyses were performed to assess different T cell subsets. Results The numbers of CD4+CD161+ cells showing either the Th1 or the Th17/Th1 phenotype were higher in the SF than in the PB of children with JIA. The few Th17 cells from JIA SF underwent a spontaneous shift to the Th1 phenotype in vitro, whereas Th17 cells from the PB of healthy children shifted only in the presence of JIA SF; this effect was neutralized by antibody blockade of IL-12 activity. Spectratyping and clonotype analyses showed a similar skewing of the T cell receptor Vβ repertoire in both CD161+ Th17 cells and CD161+ Th1 cells derived from the SF of the same JIA patient. The frequencies of CD4+CD161+ cells, particularly the Th17/Th1 cells, in the JIA SF positively correlated with the erythrocyte sedimentation rate and levels of C-reactive protein. Conclusion These findings suggest that a shifting of CD4+CD161+ T cells from Th17 to the Th17/Th1 or Th1 phenotype can occur in the SF of children with oligoarticular-onset JIA, and indicate that the accumulation of these cells is correlated with parameters of inflammation. Thus, the results support the hypothesis that these cells may play a role in JIA disease activity. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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