التفاصيل البيبلوغرافية
| العنوان: |
Construction of an Expression System for Aqualysin I in Escherichia coli That Gives a Markedly Improved Yield of the Enzyme Protein. |
| المؤلفون: |
Sakaguchi, Masayoshi, Niimiya, Keisuke, Takezawa, Makoto, Toki, Tsutomu, Sugahara, Yasusato, Kawakita, Masao |
| المصدر: |
Bioscience, Biotechnology & Biochemistry; Aug2008, Vol. 72 Issue 8, p2012-2018, 7p, 2 Charts, 4 Graphs |
| مصطلحات موضوعية: |
ESCHERICHIA coli, SERINE proteinases, GENETIC regulation, ENZYME activation, GENE expression |
| مستخلص: |
The article presents a study on the construction of an expression system for aqualysin I, alkaline serine-protease in Escherichia coli for the efficient production of enzyme protein. It says that the mature protein is being processed from a precursor through cleavage of N- and C-terminal peptide fragments. Moreover, significant protease activities were detected during the production of aqualysin I in E.coli in crude extract. |
| قاعدة البيانات: |
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