التفاصيل البيبلوغرافية
| العنوان: |
Impact of repeated bouts of eccentric exercise on myogenic gene expression. |
| المؤلفون: |
Costa, Andreas, Dalloul, Hicham, Hegyesi, Hargita, Apor, Peter, Csende, Zsolt, Racz, Levente, Vaczi, Mark, Tihanyi, Jozsef |
| المصدر: |
European Journal of Applied Physiology; Nov2007, Vol. 101 Issue 4, p427-436, 10p, 1 Chart, 5 Graphs |
| مصطلحات موضوعية: |
GENE expression, GENETIC regulation, MESSENGER RNA, DYNAMOMETER, DEHYDROGENASES, ANALYSIS of variance, MATHEMATICAL statistics, CELL differentiation, SKELETAL muscle physiology, BIOCHEMISTRY, CELL physiology, COMPARATIVE studies, CREATINE kinase, DNA, EXERCISE, GROWTH factors, LACTATE dehydrogenase, PHENOMENOLOGY, RESEARCH methodology, MEDICAL cooperation, MUSCLE contraction, MUSCLE proteins, MUSCLE strength, PHOSPHOTRANSFERASES, POLYMERASE chain reaction, RESEARCH, RNA, TUMOR markers, EVALUATION research, REVERSE transcriptase polymerase chain reaction, SKELETAL muscle, PHYSIOLOGY |
| مستخلص: |
Evidence indicates that repeated-bouts of eccentric exercise (EE) do not exacerbate the extent of muscle damage indices, as compared to a single-bout. We hypothesized that molecular adaptations, under repeated-bouts of EE, would include suppression of muscle repair inhibitory factors such as myostatin and up-regulation of muscle repair positive regulatory factors such as myogenic regulatory factors (MRFs). Fifteen males were recruited for this study. The exercise group (n=9) successfully completed six sets of 15 reps of maximum voluntary eccentric contractions, for six consecutive days, using a dynamometer (Multicont-II). Blood and muscle biopsy samples were obtained from each subject 1 week prior to exercise, 2 days post the first training session, and 24 h after the last training session. Gene expression levels were determined using real-time RT-PCR. Blood samples were analyzed for creatine kinase (CK) and lactate-dehydrogenase (LDH) activity. Repeated-bouts of EE induced a large down-regulation of myostatin mRNA (-73%) which persisted throughout the study. The responses of MRFs were mild. At day 3 only myogenin increased significantly (1.9 fold) while MyoD decreased by 45%. Surprisingly, at day 7, despite the presence of muscle damage indices, all MRFs returned to the pre-exercise levels. The results of the present study showed that repeated-bouts of EE, for six consecutive days, dramatically decreased Myostatin mRNA expression but impaired the expression patterns of MRFs such that, with the exception of myogenin that showed a moderate non-sustained increase, MyoD and MYf5 response was minimal. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
