التفاصيل البيبلوغرافية
| العنوان: |
Preparation of Calcium–Binding Peptides Derived from Mackerel (Scomber japonicus) Protein and Structural Characterization and Stability Analysis of Its Calcium Complexes. |
| المؤلفون: |
Cui, Pengbo, Liang, Jianqin, Cheng, Tianyu, Zhang, Jianyou |
| المصدر: |
Foods; Jun2024, Vol. 13 Issue 11, p1652, 13p |
| مصطلحات موضوعية: |
CYTOSKELETAL proteins, MACKERELS, CALCIUM ions, PEPTIDES, CALCIUM supplements, OXALIC acid |
| مستخلص: |
The purpose of this study was to prepare mackerel peptides (MPs) with calcium-binding capacity through an enzyme method and to investigate the potential role they play in improving the bioavailability of calcium in vitro. The calcium-binding capacity, degree of hydrolysis (DH), molecular weight (MW), and charge distribution changes with the enzymolysis time of MPs were measured. The structural characterization of mackerel peptide–calcium (MP–calcium) complexes was performed using spectroscopy and morphology analysis. The results showed that the maximum calcium-binding capacity of the obtained MPs was 120.95 mg/g when alcalase was used for 3 h, with a DH of 15.45%. Moreover, with an increase in hydrolysis time, the MW of the MPs decreased, and the negative charge increased. The carboxyl and amino groups in aspartic (Asp) and glutamate (Glu) of the MPs may act as calcium-binding sites, which are further assembled into compact nanoscale spherical complexes with calcium ions through intermolecular interactions. Furthermore, even under the influence of oxalic acid, MP–calcium complexes maintained a certain solubility. This study provides a basis for developing new calcium supplements and efficiently utilizing the mackerel protein resource. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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