التفاصيل البيبلوغرافية
| العنوان: |
Prenatal nicotine exposure leads to epigenetic alterations in peripheral nervous system signaling genes in the testis of the rat. |
| المؤلفون: |
Dali, Ouzna, Muriel-Muriel, Jose Antonio, Vargas-Baco, Ana, Tevosian, Sergei, Zubcevic, Jasenka, Smagulova, Fatima, Hayward, Linda F. |
| المصدر: |
Epigenetics & Chromatin; 5/7/2024, Vol. 17 Issue 1, p1-21, 21p |
| مصطلحات موضوعية: |
PERIPHERAL nervous system, SPERMATOGENESIS, PRENATAL exposure, TESTIS, GENE expression, PITUITARY gland, NICOTINIC receptors |
| مستخلص: |
Background: Prenatal nicotine exposure (PNE) has been documented to cause numerous deleterious effects on fetal development. However, the epigenetic changes promoted by nicotine exposure on germ cells are still not well understood. Objectives: In this study, we focused on elucidating the impact of prenatal nicotine exposure on regulatory epigenetic mechanisms important for germ cell development. Methods: Sprague-Dawley rats were exposed to nicotine during pregnancy and male progeny was analyzed at 11 weeks of age. Testis morphology was analyzed using frozen testis sections and expression of germ cell markers was examined by RT-qPCR; histone modifications were assessed by Western Blot (WB). DNA methylation analysis was performed by methylation-specific PCR of bisulfite converted DNA. Genome-wide DNA methylation was analyzed using Methylated DNA immunoprecipitation (MeDIP)-seq. We also carried out transcriptomics analysis of pituitary glands by RNA-seq. Results: We show that gestational exposure to nicotine reduces germ cell numbers, perturbs meiosis, affects the expression of germ line reprogramming responsive genes, and impacts the DNA methylation of nervous system genes in the testis. PNE also causes perturbation of gene expression in the pituitary gland of the brain. Conclusions: Our data demonstrate that PNE leads to perturbation of male spermatogenesis, and the observed effects are associated with changes of peripheral nervous system signaling pathways. Alterations in the expression of genes associated with diverse biological activities such as cell migration, cell adhesion and GABA signaling in the pituitary gland underscore the complexity of the effects of nicotine exposure during pregnancy. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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