التفاصيل البيبلوغرافية
| العنوان: |
The development of lateral flow devices for urinary biomarkers to assess kidney health. |
| المؤلفون: |
D Souza, Serena, Obeid, Wassim, Hernandez, Jeanine, Hu, David, Wen, Yumeng, Moledina, Dennis G., Albert, Andre, Gregg, Anya, Wheeler, Andrew, Philbrook, Heather Thiessen, Parikh, Chirag R. |
| المصدر: |
Scientific Reports; 4/12/2024, Vol. 14 Issue 1, p1-8, 8p |
| مصطلحات موضوعية: |
BIOMARKERS, KIDNEY physiology, KIDNEYS, INTERLEUKIN-9, UROMODULIN |
| مستخلص: |
Serum creatinine levels are insensitive to real-time changes in kidney function or injury. There is a growing interest in assessing kidney injury by measuring biomarkers in body fluid. From our previous studies, we identified and reported three urinary biomarkers namely Uromodulin (UMOD), Osteopontin (OPN), and Interleukin-9 (IL-9) to be associated with kidney health. The availability of a rapid point-of-care test for these urinary biomarkers will potentially accelerate its applicability and accessibility. In this study, we aimed to develop novel lateral flow device (LFD) for UMOD, OPN and IL-9. We tested paired antibodies using Enzyme Linked Immunosorbent Assay wherein we observed functionality only for UMOD and OPN and not for IL-9. A conjugation buffer pH of 7.8 and 8.5 was found suitable at a detection antibody concentration of 15 µg/mL for LFD development. The developed LFDs were found to quantitatively measure UMOD standard (LLOD of 80,000 pg/mL) and OPN standard (LLOD of 8600 pg/mL) respectively. The LFD was also able to measure human urinary UMOD and OPN with a percent CV of 12.12 and 5.23 respectively. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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