التفاصيل البيبلوغرافية
| العنوان: |
NAP-seq reveals multiple classes of structured noncoding RNAs with regulatory functions. |
| المؤلفون: |
Liu, Shurong, Huang, Junhong, Zhou, Jie, Chen, Siyan, Zheng, Wujian, Liu, Chang, Lin, Qiao, Zhang, Ping, Wu, Di, He, Simeng, Ye, Jiayi, Liu, Shun, Zhou, Keren, Li, Bin, Qu, Lianghu, Yang, Jianhua |
| المصدر: |
Nature Communications; 3/18/2024, Vol. 15 Issue 1, p1-21, 21p |
| مستخلص: |
Up to 80% of the human genome produces “dark matter” RNAs, most of which are noncapped RNAs (napRNAs) that frequently act as noncoding RNAs (ncRNAs) to modulate gene expression. Here, by developing a method, NAP-seq, to globally profile the full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution, we reveal diverse classes of structured ncRNAs. We discover stably expressed linear intron RNAs (sliRNAs), a class of snoRNA-intron RNAs (snotrons), a class of RNAs embedded in miRNA spacers (misRNAs) and thousands of previously uncharacterized structured napRNAs in humans and mice. These napRNAs undergo dynamic changes in response to various stimuli and differentiation stages. Importantly, we show that a structured napRNA regulates myoblast differentiation and a napRNA DINAP interacts with dyskerin pseudouridine synthase 1 (DKC1) to promote cell proliferation by maintaining DKC1 protein stability. Our approach establishes a paradigm for discovering various classes of ncRNAs with regulatory functions.The genome-wide prevalence, mechanism and function of noncapped RNAs (napRNAs) are currently poorly understood. Here, the authors develop a method called NAP-seq, to globally profile the full-length sequences of napRNAs, revealing several classes of structured noncoding RNAs. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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