التفاصيل البيبلوغرافية
| العنوان: |
Pre-clinical evaluation of the efficacy and safety of human induced pluripotent stem cell-derived cardiomyocyte patch. |
| المؤلفون: |
Miyagawa, Shigeru, Kawamura, Takuji, Ito, Emiko, Takeda, Maki, Iseoka, Hiroko, Yokoyama, Junya, Harada, Akima, Mochizuki-Oda, Noriko, Imanishi-Ochi, Yukiko, Li, Junjun, Sasai, Masao, Kitaoka, Fumiyo, Nomura, Masaki, Amano, Naoki, Takahashi, Tomoko, Dohi, Hiromi, Morii, Eiichi, Sawa, Yoshiki |
| المصدر: |
Stem Cell Research & Therapy; 3/13/2024, Vol. 15 Issue 1, p1-15, 15p |
| مصطلحات موضوعية: |
MONONUCLEAR leukocytes, HLA histocompatibility antigens, WHOLE genome sequencing, HEART failure, MYOCARDIUM, TOXICITY testing, ARRHYTHMIA |
| مستخلص: |
Background: Cell- or tissue-based regenerative therapy is an attractive approach to treat heart failure. A tissue patch that can safely and effectively repair damaged heart muscle would greatly improve outcomes for patients with heart failure. In this study, we conducted a preclinical proof-of-concept analysis of the efficacy and safety of clinical-grade human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) patches. Methods: A clinical-grade hiPSC line was established using peripheral blood mononuclear cells from a healthy volunteer that was homozygous for human leukocyte antigens. The hiPSCs were differentiated into cardiomyocytes. The obtained hiPSC-CMs were cultured on temperature-responsive culture dishes for patch fabrication. The cellular characteristics, safety, and efficacy of hiPSCs, hiPSC-CMs, and hiPSC-CM patches were analyzed. Results: The hiPSC-CMs expressed cardiomyocyte-specific genes and proteins, and electrophysiological analyses revealed that hiPSC-CMs exhibit similar properties to human primary myocardial cells. In vitro and in vivo safety studies indicated that tumorigenic cells were absent. Moreover, whole-genome and exome sequencing revealed no genomic mutations. General toxicity tests also showed no adverse events posttransplantation. A porcine model of myocardial infarction demonstrated significantly improved cardiac function and angiogenesis in response to cytokine secretion from hiPSC-CM patches. No lethal arrhythmias were observed. Conclusions: hiPSC-CM patches are promising for future translational research and may have clinical application potential for the treatment of heart failure. [ABSTRACT FROM AUTHOR] |
|
Copyright of Stem Cell Research & Therapy is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder