التفاصيل البيبلوغرافية
| العنوان: |
Manganese suppresses the development of oral leukoplakia by activating the immune response. |
| المؤلفون: |
Shi, Yujie, Su, Chongying, Ding, Tingting, Zhao, Hang, Wang, Ying, Ren, Yuan, Wu, Lanyan, Zhang, Qiyue, Liang, Jing, Sun, Silu, Wang, Jiongke, Li, Jing, Zeng, Xin |
| المصدر: |
Oral Diseases; Mar2024, Vol. 30 Issue 2, p462-476, 15p |
| مصطلحات موضوعية: |
DENDRITIC cells, REVERSE transcriptase polymerase chain reaction, FLOW cytometry, ORAL leukoplakia, CARCINOGENS, ANIMAL experimentation, WESTERN immunoblotting, MACROPHAGES, MANGANESE, CELLULAR signal transduction, ENZYME-linked immunosorbent assay, LACTATE dehydrogenase, DESCRIPTIVE statistics, T cells, MICE, KERATINOCYTES |
| مستخلص: |
Objective: Manganese ion (Mn2+) is reported to promote the antitumor immune response by activating the cGAS‐STING pathway, but it is unknown whether Mn2+ can prevent the malignant transformation of precancerous lesions. The effects of Mn2+ in treating oral leukoplakia (OLK) were explored in this work. Methods: Peripheral blood Mn analysis of the patients was performed using inductively coupled plasma atomic emission spectroscopy (ICP–AES). A coculture model of dendritic cells (DCs)/macrophages, CD8+ T cells, and dysplastic oral keratinocytes (DOKs) was employed to analyze the role and mechanism of Mn2+ in a simulated OLK immune microenvironment. Western blot, RT–PCR, flow cytometry, enzyme‐linked immunosorbent assay (ELISA), and lactate dehydrogenase (LDH) assays were adopted to detect the mechanism of Mn2+ in this model. 4‐nitroquinoline oxide (4NQO)‐induced OLK mice were used to assess the role of Mn2+ in suppressing OLK progression, and a novel Mn2+‐loaded guanosine‐tannic acid hydrogel (G‐TA@Mn2+ hydrogel) was fabricated and evaluated for its advantages in OLK therapy. Results: The content of Mn in patients' peripheral blood was negatively related to the progression of OLK. Mn2+ promoted the maturation and antigen presentation of DCs and macrophages and enhanced the activation of CD8+ T cells in the coculture model, resulting in effective killing of DOKs. Mechanistic analysis found that Mn2+ enhanced the anti‐OLK immune response by activating the cGAS‐STING pathway. Moreover, Mn2+ suppressed the development of 4NQO–induced carcinogenesis in the mouse model. In addition, the G‐TA@Mn2+ hydrogel had better anti‐OLK effects. Conclusions: Mn2+ enhanced the anti‐OLK immune response by activating the cGAS‐STING pathway, and the G‐TA@Mn2+ hydrogel is a potential novel therapeutic approach for OLK treatment. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
