التفاصيل البيبلوغرافية
| العنوان: |
Validation of an on-chip p16ink4a/Ki-67 dual immunostaining cervical cytology system using microfluidic device technology. |
| المؤلفون: |
Hashimoto, Kei, Kumagai, Tomoo, Nomura, Kyosuke, Miyagawa, Yuko, Tago, Saori, Takasaki, Kazuki, Takahashi, Yuko, Nishida, Haruka, Ichinose, Takayuki, Hirano, Mana, Hiraike, Haruko, Wada-Hiraike, Osamu, Sasajima, Yuko, Kim, Soo Hyeon, Nagasaka, Kazunori |
| المصدر: |
Scientific Reports; 11/18/2023, Vol. 13 Issue 1, p1-10, 10p |
| مصطلحات موضوعية: |
MICROFLUIDIC devices, CYTOLOGY, IMMUNOSTAINING, CERVICAL intraepithelial neoplasia, HUMAN papillomavirus, CERVICAL cancer |
| مستخلص: |
More specific screening systems for cervical cancer may become necessary as the human papillomavirus (HPV) vaccine becomes more widespread. Although p16/Ki-67 dual-staining cytology has several advantages, it requires advanced diagnostic skills. Here, we developed an automated on-chip immunostaining method using a microfluidic device. An electroactive microwell array (EMA) microfluidic device with patterned thin-film electrodes at the bottom of each microwell was used for single-cell capture by dielectrophoresis. Immunostaining and dual staining for p16/Ki-67 were performed on diagnosed liquid cytology samples using the EMA device. The numbers of p16/Ki-67 dual-stained cells captured by the EMA device were determined and compared among the cervical intraepithelial neoplasia (CIN) lesion samples. Seven normal, fifteen CIN grade 3, and seven CIN grade 2 samples were examined. The percentage of dual-positive cells was 18.6% in the CIN grade 2 samples and 23.6% in the CIN grade 3 samples. The percentages of dual-positive staining increased significantly as the severity of the cervical lesions increased. p16/Ki67 dual immunostaining using the EMA device is as sensitive as the conventional method of confirming the histopathological diagnosis of cervical samples. This system enables a quantified parallel analysis at the individual cell level. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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