التفاصيل البيبلوغرافية
| العنوان: |
Paclitaxel promotes mTOR signaling‐mediated apoptosis in esophageal cancer cells by targeting MUC20. |
| المؤلفون: |
Li, Meng, Feng, Zhen, Han, Rui, Hu, Benchuang, Zhang, Renfeng, Wang, Hui |
| المصدر: |
Thoracic Cancer; Nov2023, Vol. 14 Issue 31, p3089-3096, 8p |
| مصطلحات موضوعية: |
PROTEIN kinases, IN vitro studies, REVERSE transcriptase polymerase chain reaction, FLOW cytometry, BIOLOGICAL models, IN vivo studies, WESTERN immunoblotting, ANIMAL experimentation, APOPTOSIS, MTOR inhibitors, CELLULAR signal transduction, CELL survival, COMPARATIVE studies, GLYCOPROTEINS, RESEARCH funding, DESCRIPTIVE statistics, PACLITAXEL, CELL lines, TUMOR markers, ESOPHAGEAL tumors, MICE, PHARMACODYNAMICS |
| مستخلص: |
Background: The aim of this study was to analyze the effect of paclitaxel on the apoptosis of esophageal cancer cells in relation to MUC20. Methods: RT‐qPCR analysis, a CCK‐8 assay, western blotting, and flow cytometry were used to analyze the anticancer effects of paclitaxel treatment or OE‐MUC20 in vitro and in vivo. Results: The in vitro results showed that paclitaxel significantly induced MUC20 upregulation and that paclitaxel treatment or OE‐MUC20 significantly decreased esophageal cancer cell viability and increased mTOR signaling activation and apoptosis. In addition, PKM2, a key downstream molecule of mTOR signaling, similarly showed significant upregulation after paclitaxel treatment in cells with OE‐MUC20, and its expression was attenuated after treatment with mTOR inhibitors. In a nude mouse model, tumor growth was slow in the OE‐MUC20 group and accelerated after inhibition of mTOR signaling. Conclusion: These data suggest that MUC20 is an important target of paclitaxel in esophageal cancer and promotes apoptosis through activation of mTOR signaling. [ABSTRACT FROM AUTHOR] |
|
Copyright of Thoracic Cancer is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder